X-Chromosome inactivation in cloned mouse embryos - PubMed (original) (raw)

X-Chromosome inactivation in cloned mouse embryos

K Eggan et al. Science. 2000.

Abstract

To study whether cloning resets the epigenetic differences between the two X chromosomes of a somatic female nucleus, we monitored X inactivation in cloned mouse embryos. Both X chromosomes were active during cleavage of cloned embryos, followed by random X inactivation in the embryo proper. In the trophectoderm (TE), X inactivation was nonrandom with the inactivated X of the somatic donor being chosen for inactivation. When female embryonic stem cells with two active X chromosomes were used as donors, random X inactivation was seen in the TE and embryo. These results demonstrate that epigenetic marks can be removed and reestablished on either X chromosome during cloning. Our results also suggest that the epigenetic marks imposed on the X chromosomes during gametogenesis, responsible for normal imprinted X inactivation in the TE, are functionally equivalent to the marks imposed on the chromosomes during somatic X inactivation.

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Comment in

• Genetics. Reprogramming X inactivation.
  Clerc P, Avner P. Clerc P, et al. Science. 2000 Nov 24;290(5496):1518-9. doi: 10.1126/science.290.5496.1518. Science. 2000. PMID: 11185510

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