2-Aminoethoxydiphenyl borate modulates kinetics of intracellular Ca(2+) signals mediated by inositol 1,4,5-trisphosphate-sensitive Ca(2+) stores in single pancreatic acinar cells of mouse - PubMed (original) (raw)
. 2000 Dec;58(6):1368-74.
doi: 10.1124/mol.58.6.1368.
Affiliations
- PMID: 11093775
- DOI: 10.1124/mol.58.6.1368
2-Aminoethoxydiphenyl borate modulates kinetics of intracellular Ca(2+) signals mediated by inositol 1,4,5-trisphosphate-sensitive Ca(2+) stores in single pancreatic acinar cells of mouse
J Wu et al. Mol Pharmacol. 2000 Dec.
Abstract
Regulation of the kinetics of intracellular Ca(2+) signals with a novel, membrane-penetrable, inositol 1,4,5-trisphosphate (InsP(3)) receptor/Ca(2+) channel modulator, 2-amino-ethoxydiphenyl borate (2APB), has been investigated using patch-clamp, whole-cell recording to monitor Ca(2+)-activated Cl(-) currents in single isolated pancreatic acinar cells. 2APB itself fails to evoke a detectable current response but it dramatically changes the kinetics of agonist-induced Ca(2+) release from pulsatile spikes to long-lasting, huge Ca(2+) waves, suggesting that 2APB coordinates local Ca(2+) release to generate global Ca(2+) signals. The regulation by 2APB can be elicited by internal perfusion of InsP(3) in a concentration-dependent manner, indicating that this regulation is not mediated through membrane receptors or G protein signal transduction. The InsP(3) receptor blocker heparin, but not the ryanodine-sensitive receptor blockers ruthenium red or ryanodine, abolishes 2APB-mediated regulation of Ca(2+) release. This results also suggest that 2APB effects are mediated through InsP(3) receptors. 2APB substantially modifies single inward Cl(-) current pulse evoked by the photolytic release of caged InsP(3) but not by caged Ca(2+). These data indicate that 2APB-induced regulation is mediated neither by Ca(2+)-induced Ca(2+) release nor by affecting Cl(-) channel activity directly. We conclude that 2APB regulates the kinetics of intracellular Ca(2+) signals, represented as the change in the Ca(2+) oscillation patterns from brief pulsatile spikes to huge, long-lasting Ca(2+) waves. Moreover, this regulation seems to be mediated through InsP(3)-sensitive Ca(2+) pools. 2APB may act as a novel, useful pharmacological tool to study the genesis of intracellular Ca(2+) signals.
Similar articles
- Calcium wave propagation in pancreatic acinar cells: functional interaction of inositol 1,4,5-trisphosphate receptors, ryanodine receptors, and mitochondria.
Straub SV, Giovannucci DR, Yule DI. Straub SV, et al. J Gen Physiol. 2000 Oct;116(4):547-60. doi: 10.1085/jgp.116.4.547. J Gen Physiol. 2000. PMID: 11004204 Free PMC article. - 2-aminoethoxydiphenyl borate inhibits agonist-induced Ca2+ signals by blocking inositol trisphosphate formation in acutely dissociated mouse pancreatic acinar cells.
Wu J, Takeo T, Suga S, Kanno T, Osanai T, Mikoshiba K, Wakui M. Wu J, et al. Pflugers Arch. 2004 Sep;448(6):592-5. doi: 10.1007/s00424-004-1295-0. Epub 2004 Jun 12. Pflugers Arch. 2004. PMID: 15197598 - Inositol 1,4,5-triphosphate-evoked responses in midbrain dopamine neurons.
Morikawa H, Imani F, Khodakhah K, Williams JT. Morikawa H, et al. J Neurosci. 2000 Oct 15;20(20):RC103. doi: 10.1523/JNEUROSCI.20-20-j0003.2000. J Neurosci. 2000. PMID: 11027254 Free PMC article. - Sarcolemma agonist-induced interactions between InsP3 and ryanodine receptors in Ca2+ oscillations and waves in smooth muscle.
McCarron JG, Bradley KN, MacMillan D, Muir TC. McCarron JG, et al. Biochem Soc Trans. 2003 Oct;31(Pt 5):920-4. doi: 10.1042/bst0310920. Biochem Soc Trans. 2003. PMID: 14505449 Review. - Local calcium spiking in pancreatic acinar cells.
Petersen OH. Petersen OH. Ciba Found Symp. 1995;188:85-94; discussion 94-103. Ciba Found Symp. 1995. PMID: 7587625 Review.
Cited by
- Assessing the Potency of the Novel Tocolytics 2-APB, Glycyl-H-1152, and HC-067047 in Pregnant Human Myometrium.
Hossain MR, Tolosa JM, Young RC, Smith R, Paul JW. Hossain MR, et al. Reprod Sci. 2023 Jan;30(1):203-220. doi: 10.1007/s43032-022-01000-2. Epub 2022 Jun 17. Reprod Sci. 2023. PMID: 35715551 Free PMC article. - Role of IP3 Receptors in Shaping the Carotid Chemoreceptor Response to Hypoxia But Not to Hypercapnia in the Rat Carotid Body: An Evidence Review.
Mokashi A, Roy A, Baby SM, Mulligan EM, Lahiri S, Di Giulio C, Pokorski M. Mokashi A, et al. Adv Exp Med Biol. 2021;1289:1-25. doi: 10.1007/5584_2020_561. Adv Exp Med Biol. 2021. PMID: 32767266 - The 2-aminoethoxydiphenyl borate analog, DPB161 blocks store-operated Ca2+ entry in acutely dissociated rat submandibular cells.
Xia K, Ma Z, Shen J, Li M, Hou B, Gao M, Zhang S, Wu J. Xia K, et al. Oncotarget. 2017 Jun 27;8(37):61551-61560. doi: 10.18632/oncotarget.18623. eCollection 2017 Sep 22. Oncotarget. 2017. PMID: 28977884 Free PMC article. - Cannabinoid receptor subtype 2 (CB2R) agonist, GW405833 reduces agonist-induced Ca(2+) oscillations in mouse pancreatic acinar cells.
Huang Z, Wang H, Wang J, Zhao M, Sun N, Sun F, Shen J, Zhang H, Xia K, Chen D, Gao M, Hammer RP, Liu Q, Xi Z, Fan X, Wu J. Huang Z, et al. Sci Rep. 2016 Jul 19;6:29757. doi: 10.1038/srep29757. Sci Rep. 2016. PMID: 27432473 Free PMC article. - Effects of thrombin and thrombin receptor activation on cardiac function after acute myocardial infarction.
Gu X, Zhang X, Lu G, Li Y, Li X, Huang H, Zeng J, Tang L. Gu X, et al. Am J Transl Res. 2015 Apr 15;7(4):654-69. eCollection 2015. Am J Transl Res. 2015. PMID: 26064435 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous