Telomere maintenance by recombination in human cells - PubMed (original) (raw)
Telomere maintenance by recombination in human cells
M A Dunham et al. Nat Genet. 2000 Dec.
Abstract
Telomeres of eukaryotic chromosomes contain many tandem repeats of a G-rich sequence (for example, TTAGGG in vertebrates). In most normal human cells, telomeres shorten with each cell division, and it is proposed that this limits the number of times these cells can replicate. Telomeres may be maintained in germline cells, and in many immortalized cells and cancers, by the telomerase holoenzyme (first discovered in the ciliate Tetrahymena), which uses an RNA subunit as template for synthesis of telomeric DNA by the reverse transcriptase catalytic subunit. Some immortalized human cell lines and some tumours maintain their telomeres in the absence of any detectable telomerase activity by a mechanism referred to as alternative lengthening of telomeres (ALT). Here we show that DNA sequences are copied from telomere to telomere in an immortalized human ALT cell line, indicating that ALT occurs by means of homologous recombination and copy switching.
Comment in
- Recombination: a means to an end in human cells.
Smith S. Smith S. Nat Genet. 2000 Dec;26(4):388-9. doi: 10.1038/82492. Nat Genet. 2000. PMID: 11101826 No abstract available.
Similar articles
- Alternative lengthening of telomeres in mammalian cells.
Henson JD, Neumann AA, Yeager TR, Reddel RR. Henson JD, et al. Oncogene. 2002 Jan 21;21(4):598-610. doi: 10.1038/sj.onc.1205058. Oncogene. 2002. PMID: 11850785 Review. - A novel telomere structure in a human alternative lengthening of telomeres cell line.
Marciniak RA, Cavazos D, Montellano R, Chen Q, Guarente L, Johnson FB. Marciniak RA, et al. Cancer Res. 2005 Apr 1;65(7):2730-7. doi: 10.1158/0008-5472.CAN-04-2888. Cancer Res. 2005. PMID: 15805272 - Repression of an alternative mechanism for lengthening of telomeres in somatic cell hybrids.
Perrem K, Bryan TM, Englezou A, Hackl T, Moy EL, Reddel RR. Perrem K, et al. Oncogene. 1999 Jun 3;18(22):3383-90. doi: 10.1038/sj.onc.1202752. Oncogene. 1999. PMID: 10362359 - A human cell line that maintains telomeres in the absence of telomerase and of key markers of ALT.
Cerone MA, Autexier C, Londoño-Vallejo JA, Bacchetti S. Cerone MA, et al. Oncogene. 2005 Nov 24;24(53):7893-901. doi: 10.1038/sj.onc.1208934. Oncogene. 2005. PMID: 16116482 - Immortalized cells with no detectable telomerase activity. A review.
Reddel RR, Bryan TM, Murnane JP. Reddel RR, et al. Biochemistry (Mosc). 1997 Nov;62(11):1254-62. Biochemistry (Mosc). 1997. PMID: 9467849 Review.
Cited by
- LAP2α orchestrates alternative lengthening of telomeres suppression through telomeric heterochromatin regulation with HDAC1: unveiling a potential therapeutic target.
Wang B, Kou H, Wang Y, Zhang Q, Jiang D, Wang J, Zhao Z, Zhou Y, Zhang M, Sui L, Zhao M, Liu Y, Liu Y, Shi L, Wang F. Wang B, et al. Cell Death Dis. 2024 Oct 19;15(10):761. doi: 10.1038/s41419-024-07116-4. Cell Death Dis. 2024. PMID: 39426946 Free PMC article. - A First-in-Class High-Throughput Screen to Discover Modulators of the Alternative Lengthening of Telomeres (ALT) Pathway.
Froney MM, Cook CR, Cadiz AM, Flinter KA, Ledeboer ST, Chan B, Burris LE, Hardy BP, Pearce KH, Wardell AC, Golitz BT, Jarstfer MB, Pattenden SG. Froney MM, et al. ACS Pharmacol Transl Sci. 2024 Aug 13;7(9):2799-2819. doi: 10.1021/acsptsci.4c00251. eCollection 2024 Sep 13. ACS Pharmacol Transl Sci. 2024. PMID: 39296266 - TRF2-RAP1 represses RAD51-dependent homology-directed telomere repair by promoting BLM-mediated D-loop unwinding and inhibiting BLM-DNA2-dependent 5'-end resection.
Liang F, Rai R, Sodeinde T, Chang S. Liang F, et al. Nucleic Acids Res. 2024 Sep 9;52(16):9695-9709. doi: 10.1093/nar/gkae642. Nucleic Acids Res. 2024. PMID: 39082275 Free PMC article. - The impact of telomere length on prostate cancer aggressiveness, genomic instability and health disparities.
Huang R, Bornman MSR, Stricker PD, Simoni Brum I, Mutambirwa SBA, Jaratlerdsiri W, Hayes VM. Huang R, et al. Sci Rep. 2024 Apr 2;14(1):7706. doi: 10.1038/s41598-024-57566-1. Sci Rep. 2024. PMID: 38565642 Free PMC article. - Alternative Lengthening of Telomeres in Yeast: Old Questions and New Approaches.
Musmaker K, Wells J, Tsai MC, Comeron JM, Malkova A. Musmaker K, et al. Biomolecules. 2024 Jan 16;14(1):113. doi: 10.3390/biom14010113. Biomolecules. 2024. PMID: 38254712 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases