Insight into protein structure and protein-ligand recognition by Fourier transform infrared spectroscopy - PubMed (original) (raw)

Review

Insight into protein structure and protein-ligand recognition by Fourier transform infrared spectroscopy

C Jung. J Mol Recognit. 2000 Nov-Dec.

Abstract

An overview of the application of Fourier transform infrared spectroscopy for the analysis of the structure of proteins and protein-ligand recognition is given. The principle of the technique and of the spectra analysis is demonstrated. Spectral signal assignments to vibrational modes of the peptide chromophore, amino acid side chains, cofactors and metal ligands are summarized. Several examples for protein-ligand recognition are discussed. A particular focus is heme proteins and, as an example, studies of cytochrome P450 are reviewed. Fourier transform infrared spectroscopy in combination with the various techniques such as time-resolved and low-temperature methods, site-directed mutagenesis and isotope labeling is a helpful approach to studying protein-ligand recognition.

PubMed Disclaimer

Similar articles

• Ligand dynamics in heme proteins observed by Fourier transform infrared spectroscopy at cryogenic temperatures.
  Nienhaus K, Nienhaus GU. Nienhaus K, et al. Methods Enzymol. 2008;437:347-78. doi: 10.1016/S0076-6879(07)37018-3. Methods Enzymol. 2008. PMID: 18433637 Review.
• Ligand binding in a docking site of cytochrome C oxidase: a time-resolved step-scan Fourier transform infrared study.
  Koutsoupakis C, Soulimane T, Varotsis C. Koutsoupakis C, et al. J Am Chem Soc. 2003 Dec 3;125(48):14728-32. doi: 10.1021/ja036107e. J Am Chem Soc. 2003. PMID: 14640647
• Use of infrared spectroscopy to monitor protein structure and stability.
  Manning MC. Manning MC. Expert Rev Proteomics. 2005 Oct;2(5):731-43. doi: 10.1586/14789450.2.5.731. Expert Rev Proteomics. 2005. PMID: 16209652 Review.

Cited by

• Heat Capacity of Spider Silk-like Block Copolymers.
  Huang W, Krishnaji S, Hu X, Kaplan D, Cebe P. Huang W, et al. Macromolecules. 2011 Jul 12;44(13):5299-5309. doi: 10.1021/ma200563t. Macromolecules. 2011. PMID: 23869111 Free PMC article.
• Study on the binding behavior and functional properties of soybean protein isolate and β-carotene.
  Zhang Y, Zhao W, Xing Z, Zhu B, Hou R, Zhang J, Li T, Zhang Z, Wang H, Li Z. Zhang Y, et al. Front Nutr. 2022 Sep 8;9:984490. doi: 10.3389/fnut.2022.984490. eCollection 2022. Front Nutr. 2022. PMID: 36159458 Free PMC article.
• Phosphoenolpyruvate and Mg2+ binding to pyruvate kinase monitored by infrared spectroscopy.
  Kumar S, Barth A. Kumar S, et al. Biophys J. 2010 May 19;98(9):1931-40. doi: 10.1016/j.bpj.2009.12.4335. Biophys J. 2010. PMID: 20441757 Free PMC article.
• Multiscale design and synthesis of biomimetic gradient protein/biosilica composites for interfacial tissue engineering.
  Guo J, Li C, Ling S, Huang W, Chen Y, Kaplan DL. Guo J, et al. Biomaterials. 2017 Nov;145:44-55. doi: 10.1016/j.biomaterials.2017.08.025. Epub 2017 Aug 15. Biomaterials. 2017. PMID: 28843732 Free PMC article.
• Infrared microspectroscopy: a multiple-screening platform for investigating single-cell biochemical perturbations upon prion infection.
  Didonna A, Vaccari L, Bek A, Legname G. Didonna A, et al. ACS Chem Neurosci. 2011 Mar 16;2(3):160-74. doi: 10.1021/cn1000952. Epub 2011 Jan 11. ACS Chem Neurosci. 2011. PMID: 22778865 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley

• Other Literature Sources

  • The Lens - Patent Citations Database