Evidence for structural dissociation of two biologic actions of growth hormone - PubMed (original) (raw)
Evidence for structural dissociation of two biologic actions of growth hormone
L A Holladay et al. Biochim Biophys Acta. 1975.
Abstract
The effects of purified growth hormone and its CNBr fragments on somatomedin induction and on the stimulation of hepatic and renal ornithine decarboxylase (L-ornithine carboxylase, EC 4.1.1.17) activity in rats have been investigated. At the doses tested, none of the CNBr fragments induced somatomedin as evidenced by lack of an effect on sulfate, leucine, and thymidine incorporation into cartilage of hypophysectomized rats. However, the largest fragment, consisting of two peptides corresponding to Residues 6-124 and 150-179 linked by a disulfide bridge, stimulated both renal and hepatic ornithine decarboxylase activity in hypophysectomized rats and the activity of the hepatic enzyme in intact animals. A smaller CNBr fragment corresponding to Residues 125-149 slightly stimulated the activity of renal ornithine decarboxylase but failed to increase activity of the hepatic enzyme. A similar slight stimulation of the activity of the renal, but not the hepatic, enzyme was produced by a large carboxyl-terminal fragment (molecular weight 8000) prepared by proteolytic cleavage of partially purified ovine growth hormone. Circular dichroic spectra of the CNBr fragments demonstrated that the largest fragment retained much of the ordered secondary structure of intact growth hormone while two smaller CNBr fragments were devoid of ordered secondary structure. These observations indicate that different biological activities of growth hormone may be dissociated by fragmentation of the parent molecule.
Similar articles
- Tissue-specific stimulation of ornithine decarboxylase activity by pituitary factors immunologically related to growth hormone.
Nicholson WE, Barton RN, Holladay LA, Orth DN, Puett D. Nicholson WE, et al. Endocrinology. 1977 Feb;100(2):459-67. doi: 10.1210/endo-100-2-459. Endocrinology. 1977. PMID: 832633 - Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.
Li CH. Li CH. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878. Proc Natl Acad Sci U S A. 1975. PMID: 812085 Free PMC article. - Hormonal regulation of renal ornithine decarboxylase activity in the rat.
Nicholson WE, Levine JH, Orth DN. Nicholson WE, et al. Endocrinology. 1976 Jan;98(1):123-8. doi: 10.1210/endo-98-1-123. Endocrinology. 1976. PMID: 174886 - Isolation and somatomedin activity of bovine growth hormone fragment 87-124.
Liberti JP. Liberti JP. Biochim Biophys Acta. 1981 Jul;675(2):239-47. doi: 10.1016/0304-4165(81)90233-6. Biochim Biophys Acta. 1981. PMID: 7272336 - Regulation of polyamine biosynthesis by antizyme and some recent developments relating the induction of polyamine biosynthesis to cell growth. Review.
Canellakis ES, Kyriakidis DA, Rinehart CA Jr, Huang SC, Panagiotidis C, Fong WF. Canellakis ES, et al. Biosci Rep. 1985 Mar;5(3):189-204. doi: 10.1007/BF01119588. Biosci Rep. 1985. PMID: 3893559 Review.
Cited by
- Rate of concentration and digestion of radioactive growth hormone preparations injected in rats, as measured by the amount and nature of radioactivity in the tissues.
Retegui-Sardou LA, Scaramal LO, Dellacha JM, Paladini AC. Retegui-Sardou LA, et al. Mol Cell Biochem. 1977 Jul 5;16(2):87-96. doi: 10.1007/BF01732048. Mol Cell Biochem. 1977. PMID: 887095 No abstract available. - Effect of unilateral ureteral obstruction on renal polyamine levels in rats.
Selmeci L, Pósch E, Mosonyi J, Hársing L. Selmeci L, et al. Experientia. 1981 Jan 15;37(1):24-5. doi: 10.1007/BF01965547. Experientia. 1981. PMID: 7202664 - Somatostatin conformation: evidence for a stable intramolecular structure from circular dichroism, diffusion, and sedimentation equilibrium.
Holladay LA, Puett D. Holladay LA, et al. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1199-202. doi: 10.1073/pnas.73.4.1199. Proc Natl Acad Sci U S A. 1976. PMID: 1063402 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials