Synthesis and release of B-lymphocyte stimulator from myeloid cells - PubMed (original) (raw)
. 2001 Jan 1;97(1):198-204.
doi: 10.1182/blood.v97.1.198.
Affiliations
- PMID: 11133761
- DOI: 10.1182/blood.v97.1.198
Free article
Synthesis and release of B-lymphocyte stimulator from myeloid cells
B Nardelli et al. Blood. 2001.
Free article
Abstract
B-lymphocyte stimulator (BLyS) is a recently identified novel member of the tumor necrosis factor ligand superfamily shown to exist in a membrane-bound and soluble form. BLyS was found to be specifically expressed on cells of myeloid lineage and to selectively stimulate B-lymphocyte proliferation and immunoglobulin production. The expression of a cytokine involved in potentiation of humoral immune responses, such as BLyS, is expected to be strictly controlled. The goal of the present study was to examine regulation of BLyS levels in monocytic cells in response to cytokines and during their differentiation to macrophages and dendritic cells. The presence of BLyS on the cell surface and in the culture medium of both normal blood monocytes and on tumor cells of myelomonocytic origin was demonstrated. BLyS gene expression and levels of membrane-associated and soluble BLyS were found to be regulated by cytokines, in particular interferon (IFN)-gamma and to a lesser extent interleukin-10 (IL-10). The expression of BLyS on monocyte membranes was retained following differentiation into macrophages, but detection on the surface of monocyte-derived dendritic cells required stimulation with IFN-gamma. Both IFN-gamma and IL-10 enhanced the release of soluble BLyS that was active in B-cell proliferation assays. Cells transfected with BLyS complementary DNA mutated in a predicted cleavage site failed to release BLyS into the culture medium, thereby suggesting that soluble BLyS was derived from the membrane form. These results provide further support for an important role for BLyS expressed in myeloid cells in B-cell expansion and antibody responses.
Similar articles
- BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator.
Moore PA, Belvedere O, Orr A, Pieri K, LaFleur DW, Feng P, Soppet D, Charters M, Gentz R, Parmelee D, Li Y, Galperina O, Giri J, Roschke V, Nardelli B, Carrell J, Sosnovtseva S, Greenfield W, Ruben SM, Olsen HS, Fikes J, Hilbert DM. Moore PA, et al. Science. 1999 Jul 9;285(5425):260-3. doi: 10.1126/science.285.5425.260. Science. 1999. PMID: 10398604 - B lymphocytes from individuals with common variable immunodeficiency respond to B lymphocyte stimulator (BLyS protein) in vitro.
Stewart DM, McAvoy MJ, Hilbert DM, Nelson DL. Stewart DM, et al. Clin Immunol. 2003 Nov;109(2):137-43. doi: 10.1016/s1521-6616(03)00215-8. Clin Immunol. 2003. PMID: 14597212 - Interferon-α-induced B-lymphocyte stimulator expression and mobilization in healthy and systemic lupus erthymatosus monocytes.
López P, Scheel-Toellner D, Rodríguez-Carrio J, Caminal-Montero L, Gordon C, Suárez A. López P, et al. Rheumatology (Oxford). 2014 Dec;53(12):2249-58. doi: 10.1093/rheumatology/keu249. Epub 2014 Jun 18. Rheumatology (Oxford). 2014. PMID: 24942493 - B lymphocyte stimulator (BLyS): a therapeutic trichotomy for the treatment of B lymphocyte diseases.
Nardelli B, Moore PA, Li Y, Hilbert DM. Nardelli B, et al. Leuk Lymphoma. 2002 Jul;43(7):1367-73. doi: 10.1080/10428190290033297. Leuk Lymphoma. 2002. PMID: 12389615 Review. - Mechanism of BLyS action in B cell immunity.
Do RK, Chen-Kiang S. Do RK, et al. Cytokine Growth Factor Rev. 2002 Feb;13(1):19-25. doi: 10.1016/s1359-6101(01)00025-9. Cytokine Growth Factor Rev. 2002. PMID: 11750877 Review.
Cited by
- Cellular indexing of transcriptomes and epitopes (CITE-Seq) in hidradenitis suppurativa identifies dysregulated cell types in peripheral blood and facilitates diagnosis via machine learning.
Kumar S, Orcales F, Shih BB, Fang X, Yin C, Yates A, Dimitrion P, Neuhaus I, Johnson C, Adrianto I, Wiala A, Hamzavi I, Zhou L, Naik H, Posch C, Mi QS, Liao W. Kumar S, et al. Res Sq [Preprint]. 2024 Sep 9:rs.3.rs-4791069. doi: 10.21203/rs.3.rs-4791069/v1. Res Sq. 2024. PMID: 39315268 Free PMC article. Preprint. - Changes in monocyte subsets in volunteers who received an oral wild-type Salmonella Typhi challenge and reached typhoid diagnosis criteria.
Toapanta FR, Hu J, Shirey KA, Bernal PJ, Levine MM, Darton TC, Waddington CS, Pollard AJ, Sztein MB. Toapanta FR, et al. Front Immunol. 2024 Aug 27;15:1454857. doi: 10.3389/fimmu.2024.1454857. eCollection 2024. Front Immunol. 2024. PMID: 39263222 Free PMC article. - Serum BAFF levels are associated with the prognosis of idiopathic membranous nephropathy.
Li Z, Chen P, Zhang Y, Chen J, Zheng S, Li W, Tang L, Liu Y, Zhao N. Li Z, et al. Ren Fail. 2024 Dec;46(2):2391069. doi: 10.1080/0886022X.2024.2391069. Epub 2024 Aug 14. Ren Fail. 2024. PMID: 39143819 Free PMC article. - Unraveling the Role of the NLRP3 Inflammasome in Lymphoma: Implications in Pathogenesis and Therapeutic Strategies.
Stergiou IE, Tsironis C, Papadakos SP, Tsitsilonis OE, Dimopoulos MA, Theocharis S. Stergiou IE, et al. Int J Mol Sci. 2024 Feb 17;25(4):2369. doi: 10.3390/ijms25042369. Int J Mol Sci. 2024. PMID: 38397043 Free PMC article. Review. - Transcriptome analysis of renal ischemia/reperfusion (I/R) injury in BAFF and BAFF-R deficient mice.
Möckel T, Boegel S, Schwarting A. Möckel T, et al. PLoS One. 2023 Sep 26;18(9):e0291619. doi: 10.1371/journal.pone.0291619. eCollection 2023. PLoS One. 2023. PMID: 37751458 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources