Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms - PubMed (original) (raw)
. 2000 Dec;408(6815):936-43.
doi: 10.1038/35050030.
Affiliations
- PMID: 11140673
- DOI: 10.1038/35050030
Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms
L Chen et al. Nature. 2000 Dec.
Abstract
Stargazer, an ataxic and epileptic mutant mouse, lacks functional AMPA (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate) receptors on cerebellar granule cells. Stargazin, the mutated protein, interacts with both AMPA receptor subunits and synaptic PDZ proteins, such as PSD-95. The interaction of stargazin with AMPA receptor subunits is essential for delivering functional receptors to the surface membrane of granule cells, whereas its binding with PSD-95 and related PDZ proteins through a carboxy-terminal PDZ-binding domain is required for targeting the AMPA receptor to synapses. Expression of a mutant stargazin lacking the PDZ-binding domain in hippocampal pyramidal cells disrupts synaptic AMPA receptors, indicating that stargazin-like mechanisms for targeting AMPA receptors may be widespread in the central nervous system.
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