Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site - PubMed (original) (raw)
Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site
H Boudin et al. Neuron. 2000 Nov.
Free article
Abstract
Aggregation of neurotransmitter receptors at pre- and postsynaptic structures is crucial for efficient neuronal communication. In contrast to the wealth of information about postsynaptic specializations, little is known about the molecular organization of presynaptic membrane proteins. We show here that the metabotropic glutamate receptor mGluR7a, which localizes specifically to presynaptic active zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous systems induces coclustering dependent upon the extreme C terminus of mGluR7a and the PDZ domain of PICK1. mGluR7a and PICK1 localize to excitatory synapses in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic sites, mGluR7adelta3 lacking the PICK1 binding site targets to axons but does not cluster. These results suggest that PICK1 is a component of the presynaptic machinery involved in mGluR7a aggregation and in modulation of glutamate neurotransmission.
Similar articles
- Molecular determinants for PICK1 synaptic aggregation and mGluR7a receptor coclustering: role of the PDZ, coiled-coil, and acidic domains.
Boudin H, Craig AM. Boudin H, et al. J Biol Chem. 2001 Aug 10;276(32):30270-6. doi: 10.1074/jbc.M102991200. Epub 2001 May 24. J Biol Chem. 2001. PMID: 11375398 - PICK1 interacts with and regulates PKC phosphorylation of mGLUR7.
Dev KK, Nakajima Y, Kitano J, Braithwaite SP, Henley JM, Nakanishi S. Dev KK, et al. J Neurosci. 2000 Oct 1;20(19):7252-7. doi: 10.1523/JNEUROSCI.20-19-07252.2000. J Neurosci. 2000. PMID: 11007882 Free PMC article. - Knock-in mice lacking the PDZ-ligand motif of mGluR7a show impaired PKC-dependent autoinhibition of glutamate release, spatial working memory deficits, and increased susceptibility to pentylenetetrazol.
Zhang CS, Bertaso F, Eulenburg V, Lerner-Natoli M, Herin GA, Bauer L, Bockaert J, Fagni L, Betz H, Scheschonka A. Zhang CS, et al. J Neurosci. 2008 Aug 20;28(34):8604-14. doi: 10.1523/JNEUROSCI.0628-08.2008. J Neurosci. 2008. PMID: 18716219 Free PMC article. - Regulation of mglu(7) receptors by proteins that interact with the intracellular C-terminus.
Dev KK, Nakanishi S, Henley JM. Dev KK, et al. Trends Pharmacol Sci. 2001 Jul;22(7):355-61. doi: 10.1016/s0165-6147(00)01684-9. Trends Pharmacol Sci. 2001. PMID: 11431030 Review. - Structure and function of PICK1.
Xu J, Xia J. Xu J, et al. Neurosignals. 2006-2007;15(4):190-201. doi: 10.1159/000098482. Epub 2007 Jan 11. Neurosignals. 2006. PMID: 17215589 Review.
Cited by
- Subsynaptic mobility of presynaptic mGluR types is differentially regulated by intra- and extracellular interactions.
Bodzęta A, Berger F, MacGillavry HD. Bodzęta A, et al. Mol Biol Cell. 2022 Jul 1;33(8):ar66. doi: 10.1091/mbc.E21-10-0484. Epub 2022 May 5. Mol Biol Cell. 2022. PMID: 35511883 Free PMC article. - The adaptor protein PICK1 targets the sorting receptor SorLA.
Binkle L, Klein M, Borgmeyer U, Kuhl D, Hermey G. Binkle L, et al. Mol Brain. 2022 Feb 19;15(1):18. doi: 10.1186/s13041-022-00903-0. Mol Brain. 2022. PMID: 35183222 Free PMC article. - Fine-tuning of GPCR activity by receptor-interacting proteins.
Ritter SL, Hall RA. Ritter SL, et al. Nat Rev Mol Cell Biol. 2009 Dec;10(12):819-30. doi: 10.1038/nrm2803. Nat Rev Mol Cell Biol. 2009. PMID: 19935667 Free PMC article. Review. - PDZ binding to the BAR domain of PICK1 is elucidated by coarse-grained molecular dynamics.
He Y, Liwo A, Weinstein H, Scheraga HA. He Y, et al. J Mol Biol. 2011 Jan 7;405(1):298-314. doi: 10.1016/j.jmb.2010.10.051. Epub 2010 Nov 2. J Mol Biol. 2011. PMID: 21050858 Free PMC article. - G-protein-coupled receptors for neurotransmitter amino acids: C-terminal tails, crowded signalosomes.
El Far O, Betz H. El Far O, et al. Biochem J. 2002 Jul 15;365(Pt 2):329-36. doi: 10.1042/BJ20020481. Biochem J. 2002. PMID: 12006104 Free PMC article. Review.