DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila - PubMed (original) (raw)

DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila

M Petronczki et al. Nat Cell Biol. 2001 Jan.

Abstract

The Drosophila protein Bazooka is required for both apical-basal polarity in epithelial cells and directing asymmetric cell division in neuroblasts. Here we show that the PDZ-domain protein DmPAR-6 cooperates with Bazooka for both of these functions. DmPAR-6 colocalizes with Bazooka at the apical cell cortex of epithelial cells and neuroblasts, and binds to Bazooka in vitro. DmPAR-6 localization requires Bazooka, and mislocalization of Bazooka through overexpression redirects DmPAR-6 to ectopic sites of the cell cortex. In the absence of DmPAR-6, Bazooka fails to localize apically in neuroblasts and epithelial cells, and is distributed in the cytoplasm instead. Epithelial cells lose their apical-basal polarity in DmPAR-6 mutants, asymmetric cell divisions in neuroblasts are misorientated, and the proteins Numb and Miranda do not segregate correctly into the basal daughter cell. Bazooka and DmPAR-6 are Drosophila homologues of proteins that direct asymmetric cell division in early Caenorhabditis elegans embryos, and our results indicate that homologous protein machineries may direct this process in worms and flies.

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Comment in

• Cell polarity: the PARty expands.
  Doe CQ. Doe CQ. Nat Cell Biol. 2001 Jan;3(1):E7-9. doi: 10.1038/35050684. Nat Cell Biol. 2001. PMID: 11146637 Review.

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