Therapeutic potential of ex vivo expanded endothelial progenitor cells for myocardial ischemia - PubMed (original) (raw)
Therapeutic potential of ex vivo expanded endothelial progenitor cells for myocardial ischemia
A Kawamoto et al. Circulation. 2001.
Abstract
Background: We investigated the therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial neovascularization.
Methods and results: Peripheral blood mononuclear cells obtained from healthy human adults were cultured in EPC medium and harvested 7 days later. Myocardial ischemia was induced by ligating the left anterior descending coronary artery in male Hsd:RH-rnu (athymic nude) rats. A total of 10(6) EPCs labeled with 1,1'-dioctadecyl-1 to 3,3,3',3'-tetramethylindocarbocyanine perchlorate were injected intravenously 3 hours after the induction of myocardial ischemia. Seven days later, fluorescence-conjugated Bandeiraea simplicifolia lectin I was administered intravenously, and the rats were immediately killed. Fluorescence microscopy revealed that transplanted EPCs accumulated in the ischemic area and incorporated into foci of myocardial neovascularization. To determine the impact on left ventricular function, 5 rats (EPC group) were injected intravenously with 10(6) EPCs 3 hours after ischemia; 5 other rats (control group) received culture media. Echocardiography, performed just before and 28 days after ischemia, disclosed ventricular dimensions that were significantly smaller and fractional shortening that was significantly greater in the EPC group than in the control group by day 28. Regional wall motion was better preserved in the EPC group. After euthanization on day 28, necropsy examination disclosed that capillary density was significantly greater in the EPC group than in the control group. Moreover, the extent of left ventricular scarring was significantly less in rats receiving EPCs than in controls. Immunohistochemistry revealed capillaries that were positive for human-specific endothelial cells.
Conclusions: Ex vivo expanded EPCs incorporate into foci of myocardial neovascularization and have a favorable impact on the preservation of left ventricular function.
Similar articles
- Endothelial progenitor cells delivered into the pericardial space incorporate into areas of ischemic myocardium.
Saltzman AJ, Choi SW, Dabreo A, Baur WE, Weiss E, Nguyen K, Ishibashi F, Celestin FF, Karia DH, Pandian NG, Karas RH, Waxman S. Saltzman AJ, et al. Cardiovasc Revasc Med. 2010 Oct-Dec;11(4):241-8. doi: 10.1016/j.carrev.2009.06.003. Cardiovasc Revasc Med. 2010. PMID: 20934657 - Implantation of endothelial progenitor cells into laser-induced channels in rat ischemia hindlimb augments neovascularization.
Liu C, Sun Z, Du X, Chen X, Feng J, Jia B. Liu C, et al. Ann Vasc Surg. 2005 Mar;19(2):241-7. doi: 10.1007/s10016-004-0173-8. Ann Vasc Surg. 2005. PMID: 15782270 - Transplantation of endothelial progenitor cells for therapeutic neovascularization.
Kawamoto A, Asahara T, Losordo DW. Kawamoto A, et al. Cardiovasc Radiat Med. 2002 Jul-Dec;3(3-4):221-5. doi: 10.1016/s1522-1865(03)00082-9. Cardiovasc Radiat Med. 2002. PMID: 12974378 Review. - Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia.
Kawamoto A, Tkebuchava T, Yamaguchi J, Nishimura H, Yoon YS, Milliken C, Uchida S, Masuo O, Iwaguro H, Ma H, Hanley A, Silver M, Kearney M, Losordo DW, Isner JM, Asahara T. Kawamoto A, et al. Circulation. 2003 Jan 28;107(3):461-8. doi: 10.1161/01.cir.0000046450.89986.50. Circulation. 2003. PMID: 12551872 - Therapeutic vasculogenesis using human cord blood-derived endothelial progenitors.
Murohara T. Murohara T. Trends Cardiovasc Med. 2001 Nov;11(8):303-7. doi: 10.1016/s1050-1738(01)00128-1. Trends Cardiovasc Med. 2001. PMID: 11728877 Review.
Cited by
- CXC-chemokine receptor 4 antagonist AMD3100 promotes cardiac functional recovery after ischemia/reperfusion injury via endothelial nitric oxide synthase-dependent mechanism.
Jujo K, Ii M, Sekiguchi H, Klyachko E, Misener S, Tanaka T, Tongers J, Roncalli J, Renault MA, Thorne T, Ito A, Clarke T, Kamide C, Tsurumi Y, Hagiwara N, Qin G, Asahi M, Losordo DW. Jujo K, et al. Circulation. 2013 Jan 1;127(1):63-73. doi: 10.1161/CIRCULATIONAHA.112.099242. Epub 2012 Nov 30. Circulation. 2013. PMID: 23204107 Free PMC article. - Endothelial progenitor cells promote tumor growth and progression by enhancing new vessel formation.
Zhao X, Liu HQ, Li J, Liu XL. Zhao X, et al. Oncol Lett. 2016 Aug;12(2):793-799. doi: 10.3892/ol.2016.4733. Epub 2016 Jun 15. Oncol Lett. 2016. PMID: 27446353 Free PMC article. - Enhanced human endothelial progenitor cell adhesion and differentiation by a bioinspired multifunctional nanomatrix.
Andukuri A, Sohn YD, Anakwenze CP, Lim DJ, Brott BC, Yoon YS, Jun HW. Andukuri A, et al. Tissue Eng Part C Methods. 2013 May;19(5):375-85. doi: 10.1089/ten.TEC.2012.0312. Epub 2012 Dec 19. Tissue Eng Part C Methods. 2013. PMID: 23126402 Free PMC article. - Endothelial progenitor cells derived from Wharton's jelly of the umbilical cord reduces ischemia-induced hind limb injury in diabetic mice by inducing HIF-1α/IL-8 expression.
Shen WC, Liang CJ, Wu VC, Wang SH, Young GH, Lai IR, Chien CL, Wang SM, Wu KD, Chen YL. Shen WC, et al. Stem Cells Dev. 2013 May 1;22(9):1408-18. doi: 10.1089/scd.2012.0445. Epub 2013 Feb 15. Stem Cells Dev. 2013. PMID: 23252631 Free PMC article. - Advances in stem cell therapy for cardiovascular disease (Review).
Sun R, Li X, Liu M, Zeng Y, Chen S, Zhang P. Sun R, et al. Int J Mol Med. 2016 Jul;38(1):23-9. doi: 10.3892/ijmm.2016.2607. Epub 2016 May 25. Int J Mol Med. 2016. PMID: 27220939 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical