DNA replication-independent silencing in S. cerevisiae - PubMed (original) (raw)
DNA replication-independent silencing in S. cerevisiae
A L Kirchmaier et al. Science. 2001.
Abstract
In Saccharomyces cerevisiae, the silent mating loci are repressed by their assembly into heterochromatin. The formation of this heterochromatin requires a cell cycle event that occurs between early S phase and G(2)/M phase, which has been widely assumed to be DNA replication. To determine whether DNA replication through a silent mating-type locus, HMRa, is required for silencing to be established, we monitored heterochromatin formation at HMRa on a chromosome and on a nonreplicating extrachromosomal cassette as cells passed through S phase. Cells that passed through S phase established silencing at both the chromosomal HMRa locus and the extrachromosomal HMRa locus with equal efficiency. Thus, in contrast to the prevailing view, the establishment of silencing occurred in the absence of passage of the DNA replication fork through or near the HMR locus, but retained a cell cycle dependence.
Comment in
- Transcription. Is S phase important for transcriptional silencing?
Smith JS, Boeke JD. Smith JS, et al. Science. 2001 Jan 26;291(5504):608-9. doi: 10.1126/science.291.5504.608. Science. 2001. PMID: 11158666 No abstract available.
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