Induction and excretion of ultraviolet-induced 8-oxo-2'-deoxyguanosine and thymine dimers in vivo: implications for PUVA - PubMed (original) (raw)
Clinical Trial
Induction and excretion of ultraviolet-induced 8-oxo-2'-deoxyguanosine and thymine dimers in vivo: implications for PUVA
M S Cooke et al. J Invest Dermatol. 2001 Feb.
Free article
Abstract
Molecular epidemiology has linked ultraviolet-induced DNA damage with mutagenesis and skin carcinogenesis. Ultraviolet radiation may damage DNA in one of two ways: either directly, leading to lesions such as cyclobutane thymine dimers (T<>T), or indirectly, via photosensitizers that generate free radical species that may ultimately produce such oxidative lesions as 8-oxo-2'-deoxyguanosine. We report the results of a pilot, case control study in which seven, healthy, human volunteers (skin type II; aged 23-56 y; three male, four female) received a suberythemal dose of whole body irradiation from ultraviolet-A-emitting fluorescent tubes used in psoralen plus ultraviolet A therapy. First void, mid-stream urine samples were collected pre-exposure and daily postexposure, for up to 13 d. Analysis of urinary 8-oxo-2'-deoxyguanosine and cyclobutane thymine dimers was by competitive enzyme-linked immunosorbent assay (interassay coefficient of variation < or = 10%) and compared with a matched, control group of unirradiated individuals. A maximal increase in levels of urinary 8-oxo-2'-deoxyguanosine was seen 4 d post-ultraviolet exposure. A subsequent reduction was noted, before finally returning to baseline. Similarly, cyclobutane thymine dimer levels peaked 3 d postexposure, before returning to baseline. In contrast to the 8-oxo-2'-deoxyguanosine analysis, however, a second peak was noted at days 9-11, before again returning to baseline. This is the first report examining urinary 8-oxo-2'-deoxyguanosine and cyclobutane thymine dimers following ultraviolet exposure of healthy human subjects. This work illustrates the induction and time course for excretion of ultraviolet-induced lesions, perhaps alluding to repair and ultimately offering the potential to define psoralen plus ultraviolet A dosage regimes in terms of minimizing DNA damage and hence cancer risk.
Similar articles
- Urinary thymine dimers and 8-oxo-2'-deoxyguanosine in psoriasis.
Ahmad J, Cooke MS, Hussieni A, Evans MD, Patel K, Burd RM, Bleiker TO, Hutchinson PE, Lunec J. Ahmad J, et al. FEBS Lett. 1999 Nov 5;460(3):549-53. doi: 10.1016/s0014-5793(99)01402-7. FEBS Lett. 1999. PMID: 10556533 - Protection against pyrimidine dimers, p53, and 8-hydroxy-2'-deoxyguanosine expression in ultraviolet-irradiated human skin by sunscreens: difference between UVB + UVA and UVB alone sunscreens.
Liardet S, Scaletta C, Panizzon R, Hohlfeld P, Laurent-Applegate L. Liardet S, et al. J Invest Dermatol. 2001 Dec;117(6):1437-41. doi: 10.1046/j.0022-202x.2001.01580.x. J Invest Dermatol. 2001. PMID: 11886505 Clinical Trial. - 1α,25 dihydroxyvitamin D3 enhances cellular defences against UV-induced oxidative and other forms of DNA damage in skin.
Gordon-Thomson C, Gupta R, Tongkao-on W, Ryan A, Halliday GM, Mason RS. Gordon-Thomson C, et al. Photochem Photobiol Sci. 2012 Dec;11(12):1837-47. doi: 10.1039/c2pp25202c. Photochem Photobiol Sci. 2012. PMID: 23069805 - 1α,25-Dihydroxyvitamin D3 reduces several types of UV-induced DNA damage and contributes to photoprotection.
Song EJ, Gordon-Thomson C, Cole L, Stern H, Halliday GM, Damian DL, Reeve VE, Mason RS. Song EJ, et al. J Steroid Biochem Mol Biol. 2013 Jul;136:131-8. doi: 10.1016/j.jsbmb.2012.11.003. Epub 2012 Nov 16. J Steroid Biochem Mol Biol. 2013. PMID: 23165145 Review.
Cited by
- Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?
Halliwell B, Whiteman M. Halliwell B, et al. Br J Pharmacol. 2004 May;142(2):231-55. doi: 10.1038/sj.bjp.0705776. Br J Pharmacol. 2004. PMID: 15155533 Free PMC article. Review. - Vitamin E inhibits the UVAI induction of "light" and "dark" cyclobutane pyrimidine dimers, and oxidatively generated DNA damage, in keratinocytes.
Delinasios GJ, Karbaschi M, Cooke MS, Young AR. Delinasios GJ, et al. Sci Rep. 2018 Jan 11;8(1):423. doi: 10.1038/s41598-017-18924-4. Sci Rep. 2018. PMID: 29323251 Free PMC article. - Biomarkers of nucleic acid oxidation - A summary state-of-the-art.
Chao MR, Evans MD, Hu CW, Ji Y, Møller P, Rossner P, Cooke MS. Chao MR, et al. Redox Biol. 2021 Jun;42:101872. doi: 10.1016/j.redox.2021.101872. Epub 2021 Jan 28. Redox Biol. 2021. PMID: 33579665 Free PMC article. - Urinary 8-OHdG: A marker of oxidative stress to DNA and total antioxidant status in essential hypertension with South Indian population.
Subash P, Gurumurthy P, Sarasabharathi A, Cherian KM. Subash P, et al. Indian J Clin Biochem. 2010 Apr;25(2):127-32. doi: 10.1007/s12291-010-0024-z. Epub 2010 May 27. Indian J Clin Biochem. 2010. PMID: 23105898 Free PMC article. - Coffee Consumption and Oxidative Stress: A Review of Human Intervention Studies.
Martini D, Del Bo' C, Tassotti M, Riso P, Del Rio D, Brighenti F, Porrini M. Martini D, et al. Molecules. 2016 Jul 28;21(8):979. doi: 10.3390/molecules21080979. Molecules. 2016. PMID: 27483219 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources