Mechanical stretch augments PDGF receptor beta expression and protein tyrosine phosphorylation in pulmonary artery tissue and smooth muscle cells - PubMed (original) (raw)

Mechanical stretch augments PDGF receptor beta expression and protein tyrosine phosphorylation in pulmonary artery tissue and smooth muscle cells

Y Tanabe et al. Mol Cell Biochem. 2000 Dec.

Abstract

With regard to the mechanotransduction mechanisms of vasculature involved in hypertensive diseases, we aimed to identify tyrosine-phosphorylated proteins in pulmonary artery that responded to mechanical stress. Mechanical stretch simultaneously augmented protein-tyrosine phosphorylation in p55, p95, p105, p115, p130, p165, p180 in pulmonary artery tissue and pulmonary artery-derived smooth muscle cells (PASMC), whereas p115 and p55 were preferentially phosphorylated by the stretch in endothelial cells (PAEC). A series of experiments designed to characterize these proteins indicated that p115 and p180 were focal adhesion kinase (FAK) and platelet-derived growth factor receptor beta (PDGF-Rbeta), respectively, and that stretch augmented the surface-expression of PDGF-Rbeta in PASMC but not in PAEC. Moreover, a significant increase in the steady-state mRNA level for PDGF-Rbeta was observed in the pulmonary artery of rats with monocrotaline-induced pulmonary hypertension, where the artery should be overstretched due to increasing pulmonary arterial blood pressure. These results suggest that stretch-induced overexpression of cell-surface PDGF-Rbeta as well as augmentation of yrosine phosphorylation of proteins including FAK in PASMC might be involved in the mechanotransduction of pulmonary artery.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Science. 1993 May 21;260(5111):1124-7 - PubMed
    1. Hypertension. 1991 Jun;17(6 Pt 2):888-95 - PubMed
    1. Cytokine. 1998 Feb;10(2):82-92 - PubMed
    1. Physiol Rev. 1979 Jan;59(1):1-61 - PubMed
    1. Am J Hypertens. 1999 Dec;12(12 Pt 1-2):164S-169S - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources