Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40 - PubMed (original) (raw)
Review
Reverse genetic studies of homologous DNA recombination using the chicken B-lymphocyte line, DT40
E Sonoda et al. Philos Trans R Soc Lond B Biol Sci. 2001.
Abstract
DT40 is an avian leucosis virus-transformed chicken B-lymphocyte line which exhibits high ratios of targeted to random integration of transfected DNA constructs. This efficient targeted integration may be related to the ongoing diversification of the variable segment of the immunoglobulin gene through homologous DNA recombination-controlled gene conversion. DT40s are a convenient model system for making gene-targeted mutants. Another advantage is the relative tractability of these cells, which makes it possible to disrupt multiple genes in a single cell and to generate conditionally gene-targeted mutants including temperature-sensitive mutants. There are strong phenotypic similarities between murine and DT40 mutants of various genes involved in DNA recombination. These similarities confirm that the DT40 cell line is a reasonable model for the analysis of vertebrate DNA recombination, despite obvious concerns associated with the use of a transformed cell line, which may have certain cell-line-specific characteristics. Here we describe our studies of homologous DNA recombination in vertebrate somatic cells using reverse genetics in DT40 cells.
Similar articles
- DNA repair studies: experimental evidence in support of chicken DT40 cell line as a unique model.
Dhar PK, Sonoda E, Fujimori A, Yamashita YM, Takeda S. Dhar PK, et al. J Environ Pathol Toxicol Oncol. 2001;20(4):273-83. J Environ Pathol Toxicol Oncol. 2001. PMID: 11797836 - Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40.
Yamazoe M, Sonoda E, Hochegger H, Takeda S. Yamazoe M, et al. DNA Repair (Amst). 2004 Aug-Sep;3(8-9):1175-85. doi: 10.1016/j.dnarep.2004.03.039. DNA Repair (Amst). 2004. PMID: 15279806 Review. - Immunoglobulin gene conversion: insights from bursal B cells and the DT40 cell line.
Arakawa H, Buerstedde JM. Arakawa H, et al. Dev Dyn. 2004 Mar;229(3):458-64. doi: 10.1002/dvdy.10495. Dev Dyn. 2004. PMID: 14991701 Review. - Immunoglobulin diversification in DT40: a model for vertebrate DNA damage tolerance.
Sale JE. Sale JE. DNA Repair (Amst). 2004 Jul 2;3(7):693-702. doi: 10.1016/j.dnarep.2004.03.042. DNA Repair (Amst). 2004. PMID: 15177178 Review. - Establishment of the DNA repair-defective mutants in DT40 cells.
Ishiai M, Uchida E, Takata M. Ishiai M, et al. Methods Mol Biol. 2012;920:39-49. doi: 10.1007/978-1-61779-998-3_4. Methods Mol Biol. 2012. PMID: 22941595
Cited by
- Towards Tabula Gallus.
Yamagata M. Yamagata M. Int J Mol Sci. 2022 Jan 6;23(2):613. doi: 10.3390/ijms23020613. Int J Mol Sci. 2022. PMID: 35054796 Free PMC article. Review. - Critical roles of Rad54 in tolerance to apigenin-induced Top1-mediated DNA damage.
Zhao Z, Wu X, He F, Xiang C, Feng X, Bai X, Liu X, Zhao J, Takeda S, Qing Y. Zhao Z, et al. Exp Ther Med. 2021 May;21(5):505. doi: 10.3892/etm.2021.9936. Epub 2021 Mar 18. Exp Ther Med. 2021. PMID: 33791014 Free PMC article. - Super-resolution mapping of cellular double-strand break resection complexes during homologous recombination.
Whelan DR, Rothenberg E. Whelan DR, et al. Proc Natl Acad Sci U S A. 2021 Mar 16;118(11):e2021963118. doi: 10.1073/pnas.2021963118. Proc Natl Acad Sci U S A. 2021. PMID: 33707212 Free PMC article. - DNA Repair Biosensor-Identified DNA Damage Activities of Endophyte Extracts from Garcinia cowa.
Lerksuthirat T, Wikiniyadhanee R, Chitphuk S, Stitchantrakul W, Sampattavanich S, Jirawatnotai S, Jumpathong J, Dejsuphong D. Lerksuthirat T, et al. Biomolecules. 2020 Dec 16;10(12):1680. doi: 10.3390/biom10121680. Biomolecules. 2020. PMID: 33339185 Free PMC article. - A novel model to characterize structure and function of BRCA1.
Lin D, Izadpanah R, Braun SE, Alt E. Lin D, et al. Cell Biol Int. 2018 Jan;42(1):34-44. doi: 10.1002/cbin.10846. Epub 2017 Oct 25. Cell Biol Int. 2018. PMID: 28833843 Free PMC article.
References
- Nat Genet. 1999 Nov;23(3):359-62 - PubMed
- Genes Dev. 1999 Sep 1;13(17):2191-5 - PubMed
- Trends Genet. 2000 Feb;16(2):69-74 - PubMed
- EMBO J. 2000 Feb 1;19(3):463-71 - PubMed
- EMBO J. 2000 Apr 3;19(7):1703-10 - PubMed
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources