Complete reconstitution of clathrin basket formation with recombinant protein fragments: adaptor control of clathrin self-assembly - PubMed (original) (raw)
Complete reconstitution of clathrin basket formation with recombinant protein fragments: adaptor control of clathrin self-assembly
B Greene et al. Traffic. 2000 Jan.
Free article
Abstract
Clathrin polymerization into a polyhedral basket, surrounding budding membrane vesicles, mediates protein sorting during endocytosis and organelle biogenesis. Adaptor proteins target clathrin assembly to specific membrane sites and sequester receptors into the clathrin coat. We have reconstituted complete clathrin basket formation from recombinantly expressed fragments of clathrin and adaptors. This reconstitution reveals a hierarchy of clathrin self-assembly interactions and demonstrates that adaptors control basket formation by alignment of the distal domains of the clathrin triskelion leg through their binding to the terminal domain.
Similar articles
- Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs.
Liu SH, Wong ML, Craik CS, Brodsky FM. Liu SH, et al. Cell. 1995 Oct 20;83(2):257-67. doi: 10.1016/0092-8674(95)90167-1. Cell. 1995. PMID: 7585943 - Novel binding sites on clathrin and adaptors regulate distinct aspects of coat assembly.
Knuehl C, Chen CY, Manalo V, Hwang PK, Ota N, Brodsky FM. Knuehl C, et al. Traffic. 2006 Dec;7(12):1688-700. doi: 10.1111/j.1600-0854.2006.00499.x. Epub 2006 Oct 17. Traffic. 2006. PMID: 17052248 - Multiple interactions of auxilin 1 with clathrin and the AP-2 adaptor complex.
Scheele U, Kalthoff C, Ungewickell E. Scheele U, et al. J Biol Chem. 2001 Sep 28;276(39):36131-8. doi: 10.1074/jbc.M106511200. Epub 2001 Jul 24. J Biol Chem. 2001. PMID: 11470803 - Adaptors for clathrin-mediated traffic.
Kirchhausen T. Kirchhausen T. Annu Rev Cell Dev Biol. 1999;15:705-32. doi: 10.1146/annurev.cellbio.15.1.705. Annu Rev Cell Dev Biol. 1999. PMID: 10611976 Review. - Life of a clathrin coat: insights from clathrin and AP structures.
Edeling MA, Smith C, Owen D. Edeling MA, et al. Nat Rev Mol Cell Biol. 2006 Jan;7(1):32-44. doi: 10.1038/nrm1786. Nat Rev Mol Cell Biol. 2006. PMID: 16493411 Review.
Cited by
- Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.
Redlingshöfer L, McLeod F, Chen Y, Camus MD, Burden JJ, Palomer E, Briant K, Dannhauser PN, Salinas PC, Brodsky FM. Redlingshöfer L, et al. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23527-23538. doi: 10.1073/pnas.2003662117. Epub 2020 Sep 9. Proc Natl Acad Sci U S A. 2020. PMID: 32907943 Free PMC article. - Clathrin self-assembly involves coordinated weak interactions favorable for cellular regulation.
Wakeham DE, Chen CY, Greene B, Hwang PK, Brodsky FM. Wakeham DE, et al. EMBO J. 2003 Oct 1;22(19):4980-90. doi: 10.1093/emboj/cdg511. EMBO J. 2003. PMID: 14517237 Free PMC article. - Clathrin Assembly Regulated by Adaptor Proteins in Coarse-Grained Models.
Giani M, den Otter WK, Briels WJ. Giani M, et al. Biophys J. 2016 Jul 12;111(1):222-35. doi: 10.1016/j.bpj.2016.06.003. Biophys J. 2016. PMID: 27410749 Free PMC article. - Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments.
Hom N, Mehta KR, Chou T, Foraker AB, Brodsky FM, Kirshenbaum K, Montclare JK. Hom N, et al. J Mater Chem. 2012 Dec 28;22(44):23335-23339. doi: 10.1039/C2JM35019J. J Mater Chem. 2012. PMID: 23836951 Free PMC article. - The structures of natively assembled clathrin-coated vesicles.
Paraan M, Mendez J, Sharum S, Kurtin D, He H, Stagg SM. Paraan M, et al. Sci Adv. 2020 Jul 22;6(30):eaba8397. doi: 10.1126/sciadv.aba8397. eCollection 2020 Jul. Sci Adv. 2020. PMID: 32743076 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources