Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly - PubMed (original) (raw)
Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic-spindle assembly
R E Carazo-Salas et al. Nat Cell Biol. 2001 Mar.
Abstract
It was recently reported that GTP-bound Ran induces microtubule and pseudo-spindle assembly in mitotic egg extracts in the absence of chromosomes and centrosomes, and that chromosomes induce the assembly of spindle microtubules in these extracts through generation of Ran-GTP. Here we examine the effects of Ran-GTP on microtubule nucleation and dynamics and show that Ran-GTP has independent effects on both the nucleation activity of centrosomes and the stability of centrosomal microtubules. We also show that inhibition of Ran-GTP production, even in the presence of duplicated centrosomes and kinetochores, prevents assembly of a bipolar spindle in M-phase extracts.
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