Antisense knockout of HOXB4 blocks 1,25-dihydroxyvitamin D3 inhibition of c-myc expression - PubMed (original) (raw)
Antisense knockout of HOXB4 blocks 1,25-dihydroxyvitamin D3 inhibition of c-myc expression
Q Pan et al. J Endocrinol. 2001 Apr.
Abstract
The expression of c-myc is decreased by 1,25-(OH)2D(3) during HL-60 cell differentiation. Concomitantly, 1,25-(OH)2D(3) increases the expression and DNA binding activity of HOXB4, a homeobox gene. HOXB4 binds to the c-my c gene at sites involved in blocking c-myc transcription elongation. In this study, a phosphorothioate antisense oligonucleotide targeted against HOXB4 was examined for its effect on 1,25-(OH)2D(3) inhibition of c-myc expression. Alone, 1,25-(OH)2D(3) (20 nM) increased HOXB4 levels by 103+/-7% (mean+/-s.e., n=3) and decreased c-myc levels by 89+/-5% (mean+/-s.e.m., n=3) at 48 h of treatment. HOXB4 antisense treatment completely blocked the induction of HOXB4 by 1,25-(OH)2D(3). In addition, HOXB4 antisense partially blocked 1,25-(OH)2D(3)-mediated decrease in c-myc levels (46+/-6% inhibition) and promotion of HL-60 cell differentiation (20+/-2% and 25+/-3% inhibition as assessed by nitroblue tetrazolium and non-specific esterase assays respectively). The data further establish that HOXB4 levels are regulated by 1,25-(OH)2D(3) and reveal that HOXB4 participates in the down-regulation of c-myc expression.
Similar articles
- Antisense oligonucleotides targeted against protein kinase Cbeta and CbetaII block 1,25-(OH)2D3-induced differentiation.
Simpson RU, O'Connell TD, Pan Q, Newhouse J, Somerman MJ. Simpson RU, et al. J Biol Chem. 1998 Jul 31;273(31):19587-91. doi: 10.1074/jbc.273.31.19587. J Biol Chem. 1998. PMID: 9677384 - Capsaicin potentiates 1,25-dihydoxyvitamin D3- and all-trans retinoic acid-induced differentiation of human promyelocytic leukemia HL-60 cells.
Kang SN, Chung SW, Kim TS. Kang SN, et al. Eur J Pharmacol. 2001 May 25;420(2-3):83-90. doi: 10.1016/s0014-2999(01)00994-3. Eur J Pharmacol. 2001. PMID: 11408028 - 1Alpha,25-dihydroxyvitamin D3 inhibits programmed cell death in HL-60 cells by activation of sphingosine kinase.
Kleuser B, Cuvillier O, Spiegel S. Kleuser B, et al. Cancer Res. 1998 May 1;58(9):1817-24. Cancer Res. 1998. PMID: 9581819
Cited by
- Reduced proliferative capacity of hematopoietic stem cells deficient in Hoxb3 and Hoxb4.
Björnsson JM, Larsson N, Brun AC, Magnusson M, Andersson E, Lundström P, Larsson J, Repetowska E, Ehinger M, Humphries RK, Karlsson S. Björnsson JM, et al. Mol Cell Biol. 2003 Jun;23(11):3872-83. doi: 10.1128/MCB.23.11.3872-3883.2003. Mol Cell Biol. 2003. PMID: 12748289 Free PMC article. - Oxygen differentially affects the hox proteins Hoxb5 and Hoxa5 altering airway branching and lung vascular formation.
Silfa-Mazara F, Mujahid S, Thomas C, Vong T, Larsson I, Nielsen HC, Volpe MV. Silfa-Mazara F, et al. J Cell Commun Signal. 2014 Sep;8(3):231-44. doi: 10.1007/s12079-014-0237-7. Epub 2014 Jul 30. J Cell Commun Signal. 2014. PMID: 25073509 Free PMC article. - Unique spatial and cellular expression patterns of Hoxa5, Hoxb4, and Hoxb6 proteins in normal developing murine lung are modified in pulmonary hypoplasia.
Volpe MV, Wang KT, Nielsen HC, Chinoy MR. Volpe MV, et al. Birth Defects Res A Clin Mol Teratol. 2008 Aug;82(8):571-84. doi: 10.1002/bdra.20481. Birth Defects Res A Clin Mol Teratol. 2008. PMID: 18553509 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials