Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex - PubMed (original) (raw)

. 2001 Jun 8;276(23):20727-34.

doi: 10.1074/jbc.M100088200. Epub 2001 Feb 27.

Affiliations

• PMID: 11279001
• DOI: 10.1074/jbc.M100088200

Free article

Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex

S S Chong et al. J Biol Chem. 2001.

Free article

Abstract

The human U6 small nuclear (sn) RNA core promoter consists of a proximal sequence element, which recruits the multisubunit factor SNAP(c), and a TATA box, which recruits the TATA box-binding protein, TBP. In addition to SNAP(c) and TBP, transcription from the human U6 promoter requires two well defined factors. The first is hB", a human homologue of the B" subunit of yeast TFIIIB generally required for transcription of RNA polymerase III genes, and the second is hBRFU, one of two human homologues of the yeast TFIIIB subunit BRF specifically required for transcription of U6-type RNA polymerase III promoters. Here, we have partially purified and characterized a RNA polymerase III complex that can direct transcription from the human U6 promoter when combined with recombinant SNAP(c), recombinant TBP, recombinant hB", and recombinant hBRFU. These results open the way to reconstitution of U6 transcription from entirely defined components.

PubMed Disclaimer

Similar articles

• The symmetry of the yeast U6 RNA gene's TATA box and the orientation of the TATA-binding protein in yeast TFIIIB.
  Whitehall SK, Kassavetis GA, Geiduschek EP. Whitehall SK, et al. Genes Dev. 1995 Dec 1;9(23):2974-85. doi: 10.1101/gad.9.23.2974. Genes Dev. 1995. PMID: 7498793
• Identical components of yeast transcription factor IIIB are required and sufficient for transcription of TATA box-containing and TATA-less genes.
  Joazeiro CA, Kassavetis GA, Geiduschek EP. Joazeiro CA, et al. Mol Cell Biol. 1994 Apr;14(4):2798-808. doi: 10.1128/mcb.14.4.2798-2808.1994. Mol Cell Biol. 1994. PMID: 8139577 Free PMC article.
• Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.
  Schramm L, Pendergrast PS, Sun Y, Hernandez N. Schramm L, et al. Genes Dev. 2000 Oct 15;14(20):2650-63. doi: 10.1101/gad.836400. Genes Dev. 2000. PMID: 11040218 Free PMC article.
• Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.
  Huang Y, Maraia RJ. Huang Y, et al. Nucleic Acids Res. 2001 Jul 1;29(13):2675-90. doi: 10.1093/nar/29.13.2675. Nucleic Acids Res. 2001. PMID: 11433012 Free PMC article. Review.
• Small nuclear RNA genes: a model system to study fundamental mechanisms of transcription.
  Hernandez N. Hernandez N. J Biol Chem. 2001 Jul 20;276(29):26733-6. doi: 10.1074/jbc.R100032200. Epub 2001 Jun 4. J Biol Chem. 2001. PMID: 11390411 Review. No abstract available.

Cited by

• A structural perspective of human RNA polymerase III.
  Wang Q, Lei M, Wu J. Wang Q, et al. RNA Biol. 2022;19(1):246-255. doi: 10.1080/15476286.2021.2022293. Epub 2021 Dec 31. RNA Biol. 2022. PMID: 35133940 Free PMC article.
• MAF1 is a chronic repressor of RNA polymerase III transcription in the mouse.
  Bonhoure N, Praz V, Moir RD, Willemin G, Mange F, Moret C, Willis IM, Hernandez N. Bonhoure N, et al. Sci Rep. 2020 Jul 20;10(1):11956. doi: 10.1038/s41598-020-68665-0. Sci Rep. 2020. PMID: 32686713 Free PMC article.
• Quantifying ChIP-seq data: a spiking method providing an internal reference for sample-to-sample normalization.
  Bonhoure N, Bounova G, Bernasconi D, Praz V, Lammers F, Canella D, Willis IM, Herr W, Hernandez N, Delorenzi M; CycliX Consortium. Bonhoure N, et al. Genome Res. 2014 Jul;24(7):1157-68. doi: 10.1101/gr.168260.113. Epub 2014 Apr 7. Genome Res. 2014. PMID: 24709819 Free PMC article.
• Gene duplication and neofunctionalization: POLR3G and POLR3GL.
  Renaud M, Praz V, Vieu E, Florens L, Washburn MP, l'Hôte P, Hernandez N. Renaud M, et al. Genome Res. 2014 Jan;24(1):37-51. doi: 10.1101/gr.161570.113. Epub 2013 Oct 9. Genome Res. 2014. PMID: 24107381 Free PMC article.
• A multiplicity of factors contributes to selective RNA polymerase III occupancy of a subset of RNA polymerase III genes in mouse liver.
  Canella D, Bernasconi D, Gilardi F, LeMartelot G, Migliavacca E, Praz V, Cousin P, Delorenzi M, Hernandez N; CycliX Consortium. Canella D, et al. Genome Res. 2012 Apr;22(4):666-80. doi: 10.1101/gr.130286.111. Epub 2012 Jan 27. Genome Res. 2012. PMID: 22287103 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource