Spatial regulation of the exocyst complex by Rho1 GTPase - PubMed (original) (raw)
Spatial regulation of the exocyst complex by Rho1 GTPase
W Guo et al. Nat Cell Biol. 2001 Apr.
Abstract
Spatial regulation of membrane traffic is fundamental to many biological processes, including epithelial cell polarization and neuronal synaptogenesis. The multiprotein exocyst complex is localized to sites of polarized exocytosis, and is required for vesicle targeting and docking at specific domains of the plasma membrane. One component of the complex, Sec3, is thought to be a spatial landmark for polarized exocytosis. We have searched for proteins that regulate the polarized localization of the exocyst in the budding yeast Saccharomyces cerevisiae. Here we report that certain rho1 mutant alleles specifically affect the localization of the exocyst proteins. Sec3 interacts directly with Rho1 in its GTP-bound form, and functional Rho1 is needed both to establish and to maintain the polarized localization of Sec3. Sec3 is not the only mediator of the effect of Rho1 on the exocyst, because some members of the complex are correctly targeted independently of the interaction between Rho1 and Sec3. These results reveal the action of parallel pathways for the polarized localization of the exocytic machinery, both of which are under the control of Rho1, a master regulator of cell polarity.
Similar articles
- The Sec6/8 complex in mammalian cells: characterization of mammalian Sec3, subunit interactions, and expression of subunits in polarized cells.
Matern HT, Yeaman C, Nelson WJ, Scheller RH. Matern HT, et al. Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9648-53. doi: 10.1073/pnas.171317898. Epub 2001 Aug 7. Proc Natl Acad Sci U S A. 2001. PMID: 11493706 Free PMC article. - The exocyst complex in polarized exocytosis.
Hsu SC, TerBush D, Abraham M, Guo W. Hsu SC, et al. Int Rev Cytol. 2004;233:243-65. doi: 10.1016/S0074-7696(04)33006-8. Int Rev Cytol. 2004. PMID: 15037366 Review. - The N-terminus of Sec3 is required for cell wall integrity in yeast.
Guo Q, Duan Y, Meng N, Liu Y, Luo G. Guo Q, et al. Biochimie. 2020 Oct;177:30-39. doi: 10.1016/j.biochi.2020.07.023. Epub 2020 Aug 13. Biochimie. 2020. PMID: 32800898 - Rho GTPase regulation of exocytosis in yeast is independent of GTP hydrolysis and polarization of the exocyst complex.
Roumanie O, Wu H, Molk JN, Rossi G, Bloom K, Brennwald P. Roumanie O, et al. J Cell Biol. 2005 Aug 15;170(4):583-94. doi: 10.1083/jcb.200504108. J Cell Biol. 2005. PMID: 16103227 Free PMC article. - Exocytosis: the many masters of the exocyst.
Lipschutz JH, Mostov KE. Lipschutz JH, et al. Curr Biol. 2002 Mar 19;12(6):R212-4. doi: 10.1016/s0960-9822(02)00753-4. Curr Biol. 2002. PMID: 11909549 Review.
Cited by
- Emerging Roles of Exocyst Complex in Fungi: A Review.
Zuriegat Q, Abubakar YS, Wang Z, Chen M, Zhang J. Zuriegat Q, et al. J Fungi (Basel). 2024 Aug 28;10(9):614. doi: 10.3390/jof10090614. J Fungi (Basel). 2024. PMID: 39330374 Free PMC article. Review. - Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis.
Singh D, Liu Y, Zhu YH, Zhang S, Naegele S, Wu JQ. Singh D, et al. bioRxiv [Preprint]. 2024 Jul 12:2024.07.09.602728. doi: 10.1101/2024.07.09.602728. bioRxiv. 2024. PMID: 39026698 Free PMC article. Preprint. - Primary cilia and actin regulatory pathways in renal ciliopathies.
Kalot R, Sentell Z, Kitzler TM, Torban E. Kalot R, et al. Front Nephrol. 2024 Jan 16;3:1331847. doi: 10.3389/fneph.2023.1331847. eCollection 2023. Front Nephrol. 2024. PMID: 38292052 Free PMC article. Review. - The Regulation of Exosome Generation and Function in Physiological and Pathological Processes.
Wang Y, Xiao T, Zhao C, Li G. Wang Y, et al. Int J Mol Sci. 2023 Dec 23;25(1):255. doi: 10.3390/ijms25010255. Int J Mol Sci. 2023. PMID: 38203424 Free PMC article. Review. - Septin-mediated RhoA activation engages the exocyst complex to recruit the cilium transition zone.
Safavian D, Kim MS, Xie H, El-Zeiry M, Palander O, Dai L, Collins RF, Froese C, Shannon R, Nagata KI, Trimble WS. Safavian D, et al. J Cell Biol. 2023 Apr 3;222(4):e201911062. doi: 10.1083/jcb.201911062. Epub 2023 Mar 13. J Cell Biol. 2023. PMID: 36912772 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases