Visualising the activity of the cystine-glutamate antiporter in glial cells using antibodies to aminoadipic acid, a selectively transported substrate - PubMed (original) (raw)
. 2001 Apr 1;34(1):27-38.
doi: 10.1002/glia.1037.
Affiliations
- PMID: 11284017
- DOI: 10.1002/glia.1037
Visualising the activity of the cystine-glutamate antiporter in glial cells using antibodies to aminoadipic acid, a selectively transported substrate
D V Pow. Glia. 2001.
Abstract
The cystine-glutamate antiporter is a transport system that facilitates the uptake of cystine, concomitant with the release of glutamate. The cystine accumulated by this transporter is generally considered for use in the formation of the cysteine-containing antioxidant glutathione, which is abundant in many glial cells. This study used the simple strategy of generating an antibody to aminoadipic acid, a selective substrate for the cystine-glutamate antiporter. Stereospecific accumulation of aminoadipic acid into specific cell types in rat brain slice preparations was detected immunocytochemically. Strong accumulation was detected in astroglial cells in all brain regions studied including those in white matter tracts. Strong accumulation into radial glial cells, including the retinal Müller cells and the Bergmann glial cells was also observed. Glial accumulation was observed not only in cells within the blood brain barrier, but also outside such; anterior pituitary folliculostellate cell and intermediate lobe pituitary glial cells exhibited strong accumulation of aminoadipic acid. Interestingly, some glial cells such as the posterior pituitary glial cells (pituicytes) exhibited very little if any accumulation of aminoadipic acid. Within the brain labelling was not uniform. Particularly strong labelling was noted in some regions, such as the glial cells surrounding the CA1 pyramidal cells. By contrast, neurons never exhibited uptake of aminoadipic acid. Because cystine uptake is associated with glutamate release, it is suggested that this antiporter might contribute to release of glutamate from glial cells under some pathophysiological conditions.
Copyright 2001 Wiley-Liss, Inc.
Similar articles
- Cystine/glutamate antiporter expression in retinal Müller glial cells: implications for DL-alpha-aminoadipate toxicity.
Kato S, Ishita S, Sugawara K, Mawatari K. Kato S, et al. Neuroscience. 1993 Nov;57(2):473-82. doi: 10.1016/0306-4522(93)90080-y. Neuroscience. 1993. PMID: 7906874 - The glutathione level of retinal Müller glial cells is dependent on the high-affinity sodium-dependent uptake of glutamate.
Reichelt W, Stabel-Burow J, Pannicke T, Weichert H, Heinemann U. Reichelt W, et al. Neuroscience. 1997 Apr;77(4):1213-24. doi: 10.1016/s0306-4522(96)00509-x. Neuroscience. 1997. PMID: 9130799 - alpha-Aminoadipic acid blocks the Na(+)-dependent glutamate transport into acutely isolated Müller glial cells from guinea pig retina.
Pannicke T, Stabel J, Heinemann U, Reichelt W. Pannicke T, et al. Pflugers Arch. 1994 Nov;429(1):140-2. doi: 10.1007/BF02584041. Pflugers Arch. 1994. PMID: 7708474 - Role of retinal glial cells in neurotransmitter uptake and metabolism.
Bringmann A, Pannicke T, Biedermann B, Francke M, Iandiev I, Grosche J, Wiedemann P, Albrecht J, Reichenbach A. Bringmann A, et al. Neurochem Int. 2009 Mar-Apr;54(3-4):143-60. doi: 10.1016/j.neuint.2008.10.014. Epub 2008 Dec 9. Neurochem Int. 2009. PMID: 19114072 Review. - Müller glial cells in anuran retina.
Bringmann A, Skatchkov SN, Pannicke T, Biedermann B, Wolburg H, Orkand RK, Reichenbach A. Bringmann A, et al. Microsc Res Tech. 2000 Sep 1;50(5):384-93. doi: 10.1002/1097-0029(20000901)50:5<384::AID-JEMT7>3.0.CO;2-W. Microsc Res Tech. 2000. PMID: 10941174 Review.
Cited by
- Sex- and age-dependent contribution of System x_c_- to cognitive, sensory, and social behaviors revealed by comprehensive behavioral analyses of System x_c_- null mice.
Frare C, Pitt SK, Hewett SJ. Frare C, et al. Front Behav Neurosci. 2023 Aug 15;17:1238349. doi: 10.3389/fnbeh.2023.1238349. eCollection 2023. Front Behav Neurosci. 2023. PMID: 37649973 Free PMC article. - Metabolic partitioning in the brain and its hijacking by glioblastoma.
de Ruiter Swain J, Michalopoulou E, Noch EK, Lukey MJ, Van Aelst L. de Ruiter Swain J, et al. Genes Dev. 2023 Aug 1;37(15-16):681-702. doi: 10.1101/gad.350693.123. Epub 2023 Aug 30. Genes Dev. 2023. PMID: 37648371 Free PMC article. Review. - Tonic extracellular glutamate and ischaemia: glutamate antiporter system xc - regulates anoxic depolarization in hippocampus.
Heit BS, Chu A, Sane A, Featherstone DE, Park TJ, Larson J. Heit BS, et al. J Physiol. 2023 Feb;601(3):607-629. doi: 10.1113/JP283880. Epub 2022 Nov 30. J Physiol. 2023. PMID: 36321247 Free PMC article. - Astrocytes in Addictive Disorders.
Kruyer A, Scofield MD. Kruyer A, et al. Adv Neurobiol. 2021;26:231-254. doi: 10.1007/978-3-030-77375-5_10. Adv Neurobiol. 2021. PMID: 34888837 Free PMC article. - Activation and Role of Astrocytes in Ischemic Stroke.
Shen XY, Gao ZK, Han Y, Yuan M, Guo YS, Bi X. Shen XY, et al. Front Cell Neurosci. 2021 Nov 17;15:755955. doi: 10.3389/fncel.2021.755955. eCollection 2021. Front Cell Neurosci. 2021. PMID: 34867201 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous