Inhibition of coxsackievirus B4 replication in stably transfected cells expressing human MxA protein - PubMed (original) (raw)
. 2001 Apr 25;283(1):84-92.
doi: 10.1006/viro.2001.0877.
Affiliations
- PMID: 11312665
- DOI: 10.1006/viro.2001.0877
Free article
Inhibition of coxsackievirus B4 replication in stably transfected cells expressing human MxA protein
V Chieux et al. Virology. 2001.
Free article
Abstract
Coxsackieviruses B (CVB) (B1-B6), positive-strand RNA viruses, cause a variety of diseases. CVB4 may have a causal role in insulin-dependent diabetes mellitus. IFN-alpha inhibits CVB replication; however, the mechanism is not well known. The interferon-alpha-inducible human MxA protein exerts an antiviral activity against negative-strand RNA viruses and against Semliki Forest virus, a positive-strand RNA virus. To test the antiviral spectrum of MxA against CVB4, we took advantage of stably transfected Vero cells expressing MxA (Vero/MxA) in 98% of cells. Compared with control cells, in Vero/MxA cells, CVB4 yields were dramatically reduced and expression of the VP1 CVB protein analyzed by immunofluorescence was highly restricted. Furthermore, the accumulation of positive- and negative-strand CVB4 RNA was prevented as shown by in situ hybridization and RT-PCR. These results indicate that the antiviral activity of MxA extends to CVB4 and that its replication cycle is inhibited at an early step in Vero/MxA cells.
Copyright 2001 Academic Press.
Similar articles
- Difference in susceptibility to MxA protein between a coxsackievirus B1 isolate and prototype, impact of serial cell culture passage.
Chehadeh W, Abdulkareem HA. Chehadeh W, et al. J Med Virol. 2010 Mar;82(3):424-32. doi: 10.1002/jmv.21713. J Med Virol. 2010. PMID: 20087945 - Inhibition of human parainfluenza virus-3 replication by interferon and human MxA.
Zhao H, De BP, Das T, Banerjee AK. Zhao H, et al. Virology. 1996 Jun 15;220(2):330-8. doi: 10.1006/viro.1996.0321. Virology. 1996. PMID: 8661384 - Inhibition of hepatitis B virus replication by the interferon-inducible MxA protein.
Gordien E, Rosmorduc O, Peltekian C, Garreau F, Bréchot C, Kremsdorf D. Gordien E, et al. J Virol. 2001 Mar;75(6):2684-91. doi: 10.1128/JVI.75.6.2684-2691.2001. J Virol. 2001. PMID: 11222692 Free PMC article. - Coxsackievirus B RNA replication: lessons from poliovirus.
Sean P, Semler BL. Sean P, et al. Curr Top Microbiol Immunol. 2008;323:89-121. doi: 10.1007/978-3-540-75546-3_5. Curr Top Microbiol Immunol. 2008. PMID: 18357767 Review. - Combating coxsackievirus B infections.
Alhazmi A, Nekoua MP, Mercier A, Vergez I, Sane F, Alidjinou EK, Hober D. Alhazmi A, et al. Rev Med Virol. 2023 Jan;33(1):e2406. doi: 10.1002/rmv.2406. Epub 2022 Nov 12. Rev Med Virol. 2023. PMID: 36371612 Review.
Cited by
- The Inhibitory Effect of Resveratrol from Reynoutria japonica on MNV-1, a Human Norovirus Surrogate.
Lu F, Wang J, Song M, Dai X. Lu F, et al. Food Environ Virol. 2024 Jun;16(2):241-252. doi: 10.1007/s12560-024-09592-5. Epub 2024 Apr 3. Food Environ Virol. 2024. PMID: 38570420 - Interferon-Inducible Myxovirus Resistance Proteins: Potential Biomarkers for Differentiating Viral from Bacterial Infections.
Zav'yalov VP, Hämäläinen-Laanaya H, Korpela TK, Wahlroos T. Zav'yalov VP, et al. Clin Chem. 2019 Jun;65(6):739-750. doi: 10.1373/clinchem.2018.292391. Epub 2018 Dec 28. Clin Chem. 2019. PMID: 30593466 Free PMC article. Review. - Classical swine fever virus replicated poorly in cells from MxA transgenic pigs.
Zhao Y, Wang T, Yao L, Liu B, Teng C, Ouyang H. Zhao Y, et al. BMC Vet Res. 2016 Aug 17;12(1):169. doi: 10.1186/s12917-016-0794-5. BMC Vet Res. 2016. PMID: 27535023 Free PMC article. - Antiviral activity of Bifidobacterium adolescentis SPM1605 against Coxsackievirus B3.
Kim MJ, Lee DK, Park JE, Park IH, Seo JG, Ha NJ. Kim MJ, et al. Biotechnol Biotechnol Equip. 2014 Jul 4;28(4):681-688. doi: 10.1080/13102818.2014.945237. Epub 2014 Oct 20. Biotechnol Biotechnol Equip. 2014. PMID: 26019554 Free PMC article. Review. - Type III interferons are expressed by Coxsackievirus-infected human primary hepatocytes and regulate hepatocyte permissiveness to infection.
Lind K, Svedin E, Utorova R, Stone VM, Flodström-Tullberg M. Lind K, et al. Clin Exp Immunol. 2014 Sep;177(3):687-95. doi: 10.1111/cei.12368. Clin Exp Immunol. 2014. PMID: 24773058 Free PMC article.