Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector - PubMed (original) (raw)
Measurement of fecal sulfide using gas chromatography and a sulfur chemiluminescence detector
J K Fume et al. J Chromatogr B Biomed Sci Appl. 2001.
Abstract
We describe a simple technique to measure sulfide in fecal homogenates (or any other liquid milieu), which involves acidification followed by the G.C. measurement of H2S in a gas space equilibrated with a small quantity of homogenate. An internal standard of Zn35S added to the homogenate permits correction for incomplete recovery of H2S in the gas space. The use of a sulfur chemiluminescence detector, which specifically and sensitively responds to sulfur-containing compounds, greatly facilitates this measurement.
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