Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase - PubMed (original) (raw)
. 2001 May 3;411(6833):102-7.
doi: 10.1038/35075107.
Affiliations
- PMID: 11333986
- DOI: 10.1038/35075107
Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase
D V Bulavin et al. Nature. 2001.
Abstract
Response to genotoxic stress can be considered as a multistage process involving initiation of cell-cycle arrest and maintenance of arrest during DNA repair. Although maintenance of G2/M checkpoints is known to involve Chk1, Chk2/Rad53 and upstream components, the mechanisms involved in its initiation are less well defined. Here we report that p38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates Cdc25B at serines 309 and 361, and Cdc25C at serine 216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of Cdc25B at serine 309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. In contrast, in vivo Cdc25C binding to 14-3-3 is not affected by p38 inhibition after ultraviolet radiation. We propose that regulation of Cdc25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Similar articles
- Rapid activation of G2/M checkpoint after hypertonic stress in renal inner medullary epithelial (IME) cells is protective and requires p38 kinase.
Dmitrieva NI, Bulavin DV, Fornace AJ Jr, Burg MB. Dmitrieva NI, et al. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):184-9. doi: 10.1073/pnas.231623498. Epub 2001 Dec 26. Proc Natl Acad Sci U S A. 2002. PMID: 11756692 Free PMC article. - Stress-activated mitogen-activated protein kinases c-Jun NH2-terminal kinase and p38 target Cdc25B for degradation.
Uchida S, Yoshioka K, Kizu R, Nakagama H, Matsunaga T, Ishizaka Y, Poon RY, Yamashita K. Uchida S, et al. Cancer Res. 2009 Aug 15;69(16):6438-44. doi: 10.1158/0008-5472.CAN-09-0869. Epub 2009 Jul 28. Cancer Res. 2009. PMID: 19638579 - Phosphorylation of CDC25C by AMP-activated protein kinase mediates a metabolic checkpoint during cell-cycle G2/M-phase transition.
Shen Y, Sherman JW, Chen X, Wang R. Shen Y, et al. J Biol Chem. 2018 Apr 6;293(14):5185-5199. doi: 10.1074/jbc.RA117.001379. Epub 2018 Feb 21. J Biol Chem. 2018. PMID: 29467227 Free PMC article. - p38 and Chk1 kinases: different conductors for the G(2)/M checkpoint symphony.
Bulavin DV, Amundson SA, Fornace AJ. Bulavin DV, et al. Curr Opin Genet Dev. 2002 Feb;12(1):92-7. doi: 10.1016/s0959-437x(01)00270-2. Curr Opin Genet Dev. 2002. PMID: 11790561 Review. - G2 cell cycle arrest--a direct link between PKA and Cdc25C.
Shibuya EK. Shibuya EK. Cell Cycle. 2003 Jan-Feb;2(1):39-41. doi: 10.4161/cc.2.1.291. Cell Cycle. 2003. PMID: 12695686 Review. No abstract available.
Cited by
- The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3.
Han Z, Wang R, Chi P, Zhang Z, Min L, Jiao H, Ou G, Zhou D, Qin D, Xu C, Gao Z, Qi Q, Li J, Lu Y, Wang X, Chen J, Yu X, Hu H, Li L, Deng D. Han Z, et al. Nat Commun. 2024 Oct 15;15(1):8887. doi: 10.1038/s41467-024-53277-3. Nat Commun. 2024. PMID: 39406751 Free PMC article. - CDK4/6 activity is required during G2 arrest to prevent stress-induced endoreplication.
McKenney C, Lendner Y, Guerrero Zuniga A, Sinha N, Veresko B, Aikin TJ, Regot S. McKenney C, et al. Science. 2024 May 3;384(6695):eadi2421. doi: 10.1126/science.adi2421. Epub 2024 May 3. Science. 2024. PMID: 38696576 - Evidence that Alzheimer's Disease Is a Disease of Competitive Synaptic Plasticity Gone Awry.
Huang Z. Huang Z. J Alzheimers Dis. 2024;99(2):447-470. doi: 10.3233/JAD-240042. J Alzheimers Dis. 2024. PMID: 38669548 Free PMC article. Review. - Elaeagnus umbellata Fruit Extract Protects Skin from Ultraviolet-Mediated Photoaging in Hairless Mice.
Park SM, Jung CJ, Lee DG, Yu YE, Ku TH, Hong MS, Lim TK, Paeng KI, Cho HK, Cho IJ, Ku SK. Park SM, et al. Antioxidants (Basel). 2024 Feb 3;13(2):195. doi: 10.3390/antiox13020195. Antioxidants (Basel). 2024. PMID: 38397793 Free PMC article. - FGF19 induces the cell cycle arrest at G2-phase in chondrocytes.
Chen H, Li J, Pi C, Guo D, Zhang D, Zhou X, Xie J. Chen H, et al. Cell Death Discov. 2023 Jul 15;9(1):250. doi: 10.1038/s41420-023-01543-6. Cell Death Discov. 2023. PMID: 37454120 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous