Mutations in the SAM domain of STE50 differentially influence the MAPK-mediated pathways for mating, filamentous growth and osmotolerance in Saccharomyces cerevisiae - PubMed (original) (raw)
. 2001 Mar;265(1):102-17.
doi: 10.1007/s004380000394.
Affiliations
- PMID: 11370856
- DOI: 10.1007/s004380000394
Mutations in the SAM domain of STE50 differentially influence the MAPK-mediated pathways for mating, filamentous growth and osmotolerance in Saccharomyces cerevisiae
G Jansen et al. Mol Genet Genomics. 2001 Mar.
Abstract
In Saccharomyces cerevisiae, the MAPKKK Ste11p is involved in three mitogen-activated protein kinase (MAPK) pathways required for mating, filamentous growth and the SHO1-dependent response to hyperosmolarity. All three pathways are also dependent on Ste50p. Ste50p and Ste11p interact constitutively via their N-terminal regions, which include putative SAM domains. Here we show that the interaction of Ste50p and Ste11p is differentially required for modulation of Ste11p function during mating, filamentous growth and the SHO1-dependent response to hyperosmolarity. Two derivatives of Ste50p with mutations in the SAM domain were isolated and characterised. The mutant Ste50 proteins showed reduced binding to Ste11p and a tendency to form homodimers in two-hybrid and in vitro binding assays. Interestingly, these two Ste50p-SAM mutants were associated with increased activation of the mating and filamentous-growth pathways, but a reduction in the SHO1-dependent growth response to hyperosmolarity, relative to the wild-type Ste50p. Moreover, when exposed to hyperosmolarity, these Ste50p-SAM mutants activate genes in the mating (FUS1) and filamentous-growth (FLO11) pathways to higher levels than does the wild type. Thus the Ste50p-Ste11p interaction may differentially modulate the flow of information through the various MAPK-mediated pathways.
Similar articles
- Functional characterization of the interaction of Ste50p with Ste11p MAPKKK in Saccharomyces cerevisiae.
Wu C, Leberer E, Thomas DY, Whiteway M. Wu C, et al. Mol Biol Cell. 1999 Jul;10(7):2425-40. doi: 10.1091/mbc.10.7.2425. Mol Biol Cell. 1999. PMID: 10397774 Free PMC article. - Ste50p is involved in regulating filamentous growth in the yeast Saccharomyces cerevisiae and associates with Ste11p.
Ramezani Rad M, Jansen G, Bühring F, Hollenberg CP. Ramezani Rad M, et al. Mol Gen Genet. 1998 Jul;259(1):29-38. doi: 10.1007/s004380050785. Mol Gen Genet. 1998. PMID: 9738877 - Phosphorylation of the MAPKKK regulator Ste50p in Saccharomyces cerevisiae: a casein kinase I phosphorylation site is required for proper mating function.
Wu C, Arcand M, Jansen G, Zhong M, Iouk T, Thomas DY, Meloche S, Whiteway M. Wu C, et al. Eukaryot Cell. 2003 Oct;2(5):949-61. doi: 10.1128/EC.2.5.949-961.2003. Eukaryot Cell. 2003. PMID: 14555477 Free PMC article. - The role of adaptor protein Ste50-dependent regulation of the MAPKKK Ste11 in multiple signalling pathways of yeast.
Ramezani-Rad M. Ramezani-Rad M. Curr Genet. 2003 Jun;43(3):161-70. doi: 10.1007/s00294-003-0383-6. Epub 2003 Mar 11. Curr Genet. 2003. PMID: 12764668 Review. - Signal transduction cascades regulating pseudohyphal differentiation of Saccharomyces cerevisiae.
Pan X, Harashima T, Heitman J. Pan X, et al. Curr Opin Microbiol. 2000 Dec;3(6):567-72. doi: 10.1016/s1369-5274(00)00142-9. Curr Opin Microbiol. 2000. PMID: 11121775 Review.
Cited by
- Functions for Cdc42p BEM adaptors in regulating a differentiation-type MAP kinase pathway.
Basu S, González B, Li B, Kimble G, Kozminski KG, Cullen PJ. Basu S, et al. Mol Biol Cell. 2020 Mar 15;31(6):491-510. doi: 10.1091/mbc.E19-08-0441. Epub 2020 Jan 15. Mol Biol Cell. 2020. PMID: 31940256 Free PMC article. - Proteome-wide signatures of function in highly diverged intrinsically disordered regions.
Zarin T, Strome B, Nguyen Ba AN, Alberti S, Forman-Kay JD, Moses AM. Zarin T, et al. Elife. 2019 Jul 2;8:e46883. doi: 10.7554/eLife.46883. Elife. 2019. PMID: 31264965 Free PMC article. - The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain.
Sharmeen N, Sulea T, Whiteway M, Wu C. Sharmeen N, et al. Mol Biol Cell. 2019 Mar 15;30(6):794-807. doi: 10.1091/mbc.E18-11-0708. Epub 2019 Jan 16. Mol Biol Cell. 2019. PMID: 30650049 Free PMC article. - Selection maintains signaling function of a highly diverged intrinsically disordered region.
Zarin T, Tsai CN, Nguyen Ba AN, Moses AM. Zarin T, et al. Proc Natl Acad Sci U S A. 2017 Feb 21;114(8):E1450-E1459. doi: 10.1073/pnas.1614787114. Epub 2017 Feb 6. Proc Natl Acad Sci U S A. 2017. PMID: 28167781 Free PMC article. - Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway.
Pitoniak A, Chavel CA, Chow J, Smith J, Camara D, Karunanithi S, Li B, Wolfe KH, Cullen PJ. Pitoniak A, et al. Mol Cell Biol. 2015 Jan;35(2):417-36. doi: 10.1128/MCB.00850-14. Epub 2014 Nov 10. Mol Cell Biol. 2015. PMID: 25384973 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases