Development of a novel helicase assay using electrochemiluminescence - PubMed (original) (raw)
Comparative Study
. 2001 Jun 1;293(1):31-7.
doi: 10.1006/abio.2001.5093.
Affiliations
- PMID: 11373075
- DOI: 10.1006/abio.2001.5093
Comparative Study
Development of a novel helicase assay using electrochemiluminescence
L Zhang et al. Anal Biochem. 2001.
Abstract
DNA helicases are ubiquitous enzymes involved in DNA replication, recombination, and repair. These enzymes use the energy of ATP to unwind duplex DNA. A common approach to measure DNA helicase activity utilizes electrophoresis to visualize product formation in a gel. The present study develops a rapid helicase assay based on electrochemiluminescence. The assay is adaptable to rapid high-throughput screening for chemical inhibitors. The assay is applied to Escherichia coli DnaB. Three other technologies have also been applied to DnaB and are compared to results of the electrochemiluminescence-based assay.
Copyright 2001 Academic Press.
Similar articles
- Homogenous assays for Escherichia coli DnaB-stimulated DnaG primase and DnaB helicase and their use in screening for chemical inhibitors.
Zhang Y, Yang F, Kao YC, Kurilla MG, Pompliano DL, Dicker IB. Zhang Y, et al. Anal Biochem. 2002 May 15;304(2):174-9. doi: 10.1006/abio.2002.5627. Anal Biochem. 2002. PMID: 12009693 - High-throughput screening assay for helicase enzymes.
Sivaraja M, Giordano H, Peterson MG. Sivaraja M, et al. Anal Biochem. 1998 Dec 1;265(1):22-7. doi: 10.1006/abio.1998.2875. Anal Biochem. 1998. PMID: 9866703 - Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks.
Jones JM, Nakai H. Jones JM, et al. J Mol Biol. 2001 Oct 5;312(5):935-47. doi: 10.1006/jmbi.2001.4930. J Mol Biol. 2001. PMID: 11580240 - DNA helicases of Escherichia coli.
Matson SW. Matson SW. Prog Nucleic Acid Res Mol Biol. 1991;40:289-326. doi: 10.1016/s0079-6603(08)60845-4. Prog Nucleic Acid Res Mol Biol. 1991. PMID: 1851571 Review.
Cited by
- A Graphene-Based Platform for the Assay of Duplex-DNA Unwinding by Helicase.
Jang H, Kim YK, Kwon HM, Yeo WS, Kim DE, Min DH. Jang H, et al. Angew Chem Weinheim Bergstr Ger. 2010 Aug 2;122(33):5839-5843. doi: 10.1002/ange.201001332. Epub 2010 Jul 7. Angew Chem Weinheim Bergstr Ger. 2010. PMID: 32313315 Free PMC article. - Optimization of a novel potent and selective bacterial DNA helicase inhibitor scaffold from a high throughput screening hit.
Li B, Pai R, Aiello D, Di M, Barnes MH, Peet NP, Bowlin TL, Moir DT. Li B, et al. Bioorg Med Chem Lett. 2013 Jun 15;23(12):3481-6. doi: 10.1016/j.bmcl.2013.04.055. Epub 2013 Apr 30. Bioorg Med Chem Lett. 2013. PMID: 23664213 Free PMC article. - Discovering new medicines targeting helicases: challenges and recent progress.
Shadrick WR, Ndjomou J, Kolli R, Mukherjee S, Hanson AM, Frick DN. Shadrick WR, et al. J Biomol Screen. 2013 Aug;18(7):761-81. doi: 10.1177/1087057113482586. Epub 2013 Mar 27. J Biomol Screen. 2013. PMID: 23536547 Free PMC article. Review. - A new helicase assay based on graphene oxide for anti-viral drug development.
Jang H, Ryoo SR, Lee MJ, Han SW, Min DH. Jang H, et al. Mol Cells. 2013 Apr;35(4):269-73. doi: 10.1007/s10059-013-0066-1. Epub 2013 Mar 11. Mol Cells. 2013. PMID: 23483279 Free PMC article. Review. - Coumarin-based inhibitors of Bacillus anthracis and Staphylococcus aureus replicative DNA helicase: chemical optimization, biological evaluation, and antibacterial activities.
Li B, Pai R, Di M, Aiello D, Barnes MH, Butler MM, Tashjian TF, Peet NP, Bowlin TL, Moir DT. Li B, et al. J Med Chem. 2012 Dec 27;55(24):10896-908. doi: 10.1021/jm300922h. Epub 2012 Dec 11. J Med Chem. 2012. PMID: 23231076 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources