Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus - PubMed (original) (raw)
Review
Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus
S K Mazmanian et al. Mol Microbiol. 2001 Jun.
Free article
Abstract
Many surface proteins of Gram-positive bacteria are anchored to the cell wall envelope by a transpeptidation mechanism, requiring a C-terminal sorting signal with a conserved LPXTG motif. Sortase, a membrane protein of Staphylococcus aureus, cleaves polypeptides between the threonine and the glycine of the LPXTG motif and catalyses the formation of an amide bond between the carboxyl-group of threonine and the amino-group of peptidoglycan cross-bridges. S. aureus mutants lacking the srtA gene fail to anchor and display some surface proteins and are impaired in the ability to cause animal infections. Sortase acts on surface proteins that are initiated into the secretion (Sec) pathway and have their signal peptide removed by signal peptidase. The S. aureus genome encodes two sets of sortase and secretion genes. It is conceivable that S. aureus has evolved more than one pathway for the transport of 20 surface proteins to the cell wall envelope.
Similar articles
- Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring.
Perry AM, Ton-That H, Mazmanian SK, Schneewind O. Perry AM, et al. J Biol Chem. 2002 May 3;277(18):16241-8. doi: 10.1074/jbc.M109194200. Epub 2002 Feb 20. J Biol Chem. 2002. PMID: 11856734 - Anchoring of surface proteins to the cell wall of Staphylococcus aureus. A conserved arginine residue is required for efficient catalysis of sortase A.
Marraffini LA, Ton-That H, Zong Y, Narayana SV, Schneewind O. Marraffini LA, et al. J Biol Chem. 2004 Sep 3;279(36):37763-70. doi: 10.1074/jbc.M405282200. Epub 2004 Jul 6. J Biol Chem. 2004. PMID: 15247224 - Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall.
Mazmanian SK, Liu G, Ton-That H, Schneewind O. Mazmanian SK, et al. Science. 1999 Jul 30;285(5428):760-3. doi: 10.1126/science.285.5428.760. Science. 1999. PMID: 10427003 - Protein sorting to the cell wall envelope of Gram-positive bacteria.
Ton-That H, Marraffini LA, Schneewind O. Ton-That H, et al. Biochim Biophys Acta. 2004 Nov 11;1694(1-3):269-78. doi: 10.1016/j.bbamcr.2004.04.014. Biochim Biophys Acta. 2004. PMID: 15546671 Review. - Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria.
Schneewind O, Missiakas D. Schneewind O, et al. Biochim Biophys Acta. 2014 Aug;1843(8):1687-97. doi: 10.1016/j.bbamcr.2013.11.009. Epub 2013 Nov 22. Biochim Biophys Acta. 2014. PMID: 24269844 Free PMC article. Review.
Cited by
- Structural and functional insights of sortases and their interactions with antivirulence compounds.
Sivaramalingam SS, Jothivel D, Govindarajan DK, Kadirvelu L, Sivaramakrishnan M, Chithiraiselvan DD, Kandaswamy K. Sivaramalingam SS, et al. Curr Res Struct Biol. 2024 Jun 12;8:100152. doi: 10.1016/j.crstbi.2024.100152. eCollection 2024. Curr Res Struct Biol. 2024. PMID: 38989133 Free PMC article. Review. - Halenaquinol Blocks Staphylococcal Protein A Anchoring on Cell Wall Surface by Inhibiting Sortase A in Staphylococcus aureus.
Lee J, Choi JH, Lee J, Cho E, Lee YJ, Lee HS, Oh KB. Lee J, et al. Mar Drugs. 2024 Jun 10;22(6):266. doi: 10.3390/md22060266. Mar Drugs. 2024. PMID: 38921577 Free PMC article. - Echinacoside, a promising sortase A inhibitor, combined with vancomycin against murine models of MRSA-induced pneumonia.
Jiang T, Yuan D, Wang R, Zhao C, Xu Y, Liu Y, Song W, Su X, Wang B. Jiang T, et al. Med Microbiol Immunol. 2023 Dec;212(6):421-435. doi: 10.1007/s00430-023-00782-9. Epub 2023 Oct 5. Med Microbiol Immunol. 2023. PMID: 37796314 - Exploring the modulatory impact of isosakuranetin on Staphylococcus aureus: Inhibition of sortase A activity and α-haemolysin expression.
Tian L, Wang L, Yang F, Zhou T, Jiang H. Tian L, et al. Virulence. 2023 Dec;14(1):2260675. doi: 10.1080/21505594.2023.2260675. Epub 2023 Sep 28. Virulence. 2023. PMID: 37733916 Free PMC article. - Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing.
Bapat RA, Libat R, Yuin OS, Parolia A, Ilyas MS, Khan AS, Kay MK, Pichika MR, Saxena K, Seow LL, Sidhu P, Daood U. Bapat RA, et al. Heliyon. 2023 Aug 19;9(8):e19282. doi: 10.1016/j.heliyon.2023.e19282. eCollection 2023 Aug. Heliyon. 2023. PMID: 37664740 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases