SUT1p interaction with Cyc8p(Ssn6p) relieves hypoxic genes from Cyc8p-Tup1p repression in Saccharomyces cerevisiae - PubMed (original) (raw)
SUT1p interaction with Cyc8p(Ssn6p) relieves hypoxic genes from Cyc8p-Tup1p repression in Saccharomyces cerevisiae
M Régnacq et al. Mol Microbiol. 2001 Jun.
Free article
Abstract
SUT1 is a hypoxic gene encoding a nuclear protein that belongs to the Zn[II]2Cys-6 family. It has been shown that constitutive expression of SUT1 induces exogenous sterol uptake in aerobically growing Saccharomyces cerevisiae cells. A differential display approach was used to identify genes whose transcription is modified upon SUT1 induction. Within the promoter sequence of one of these genes, DAN1, we identified the region responsive to SUT1 and showed that it has a strong repressive activity when cloned in the vicinity of distinct promoters. Upon SUT1 constitutive expression in aerobiosis, the repression is released, allowing enhanced transcription of the reporter gene. We provide evidence that the repression is promoted by the Cyc8p(Ssn6p)-Tup1p co-repressor and that release of repression is the result of a physical interaction between Sut1p and Cyc8p. Moreover, genetic data suggest that complete derepression of the reporter gene requires a functional Cyc8p. In addition, we show that Sut1p is involved in the induction of hypoxic gene transcription when the cells are shifted from aerobiosis to anaerobiosis.
Similar articles
- Activator Gcn4p and Cyc8p/Tup1p are interdependent for promoter occupancy at ARG1 in vivo.
Kim SJ, Swanson MJ, Qiu H, Govind CK, Hinnebusch AG. Kim SJ, et al. Mol Cell Biol. 2005 Dec;25(24):11171-83. doi: 10.1128/MCB.25.24.11171-11183.2005. Mol Cell Biol. 2005. PMID: 16314536 Free PMC article. - SUT1-promoted sterol uptake involves the ABC transporter Aus1 and the mannoprotein Dan1 whose synergistic action is sufficient for this process.
Alimardani P, Régnacq M, Moreau-Vauzelle C, Ferreira T, Rossignol T, Blondin B, Bergès T. Alimardani P, et al. Biochem J. 2004 Jul 1;381(Pt 1):195-202. doi: 10.1042/BJ20040297. Biochem J. 2004. PMID: 15035656 Free PMC article. - Diverse roles of Tup1p and Cyc8p transcription regulators in the development of distinct types of yeast populations.
Váchová L, Palková Z. Váchová L, et al. Curr Genet. 2019 Feb;65(1):147-151. doi: 10.1007/s00294-018-0883-z. Epub 2018 Sep 6. Curr Genet. 2019. PMID: 30191307 Review. - Rox1 mediated repression. Oxygen dependent repression in yeast.
Kastaniotis AJ, Zitomer RS. Kastaniotis AJ, et al. Adv Exp Med Biol. 2000;475:185-95. Adv Exp Med Biol. 2000. PMID: 10849660 Review.
Cited by
- The influence of flocculation upon global gene transcription in a yeast CYC8 mutant.
Lee B, Hokamp K, Alhussain MM, Bamagoos AA, Fleming AB. Lee B, et al. Microb Genom. 2024 Mar;10(3):001216. doi: 10.1099/mgen.0.001216. Microb Genom. 2024. PMID: 38529898 Free PMC article. - Adaptive Response of Saccharomyces Hosts to Totiviridae L-A dsRNA Viruses Is Achieved through Intrinsically Balanced Action of Targeted Transcription Factors.
Ravoitytė B, Lukša J, Wellinger RE, Serva S, Servienė E. Ravoitytė B, et al. J Fungi (Basel). 2022 Apr 9;8(4):381. doi: 10.3390/jof8040381. J Fungi (Basel). 2022. PMID: 35448612 Free PMC article. - Regulated repression governs the cell fate promoter controlling yeast meiosis.
Tam J, van Werven FJ. Tam J, et al. Nat Commun. 2020 May 8;11(1):2271. doi: 10.1038/s41467-020-16107-w. Nat Commun. 2020. PMID: 32385261 Free PMC article. - Predictive models of eukaryotic transcriptional regulation reveals changes in transcription factor roles and promoter usage between metabolic conditions.
Holland P, Bergenholm D, Börlin CS, Liu G, Nielsen J. Holland P, et al. Nucleic Acids Res. 2019 Jun 4;47(10):4986-5000. doi: 10.1093/nar/gkz253. Nucleic Acids Res. 2019. PMID: 30976803 Free PMC article. - Archetypal transcriptional blocks underpin yeast gene regulation in response to changes in growth conditions.
Talavera D, Kershaw CJ, Costello JL, Castelli LM, Rowe W, Sims PFG, Ashe MP, Grant CM, Pavitt GD, Hubbard SJ. Talavera D, et al. Sci Rep. 2018 May 21;8(1):7949. doi: 10.1038/s41598-018-26170-5. Sci Rep. 2018. PMID: 29785040 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases