Heparanase expression in primary and metastatic pancreatic cancer - PubMed (original) (raw)

. 2001 Jun 15;61(12):4655-9.

Affiliations

PMID: 11406531

Heparanase expression in primary and metastatic pancreatic cancer

A Koliopanos et al. Cancer Res. 2001.

Abstract

The human endoglycosidase heparanase (hpa) degrades heparan-sulfate proteoglycans, which constitute prominent components of basement membranes and extracellular matrix. Due to the critical function of hpa in cancer cell invasion and metastasis, we have analyzed the expression of hpa in human primary and metastatic pancreatic cancer as well as in the normal pancreas and in chronic pancreatic inflammation. By real-time quantitative PCR, there was a 7.9- and 30.2-fold increase of hpa mRNA in chronic pancreatitis and pancreatic cancer tissue samples, respectively, in comparison with normal pancreatic tissues. There was a significant correlation between enhanced hpa mRNA expression and shorter postoperative patient survival. hpa mRNA and protein localized in the cancer cells of primary and metastatic pancreatic cancer, with a preferentially higher expression at the primary tumor site. Cultured pancreatic cancer cells transfected with a full-length hpa construct displayed enhanced invasiveness in an invasion chamber assay. These results suggest that hpa overexpression in human pancreatic cancers facilitates cancer cell invasion, thereby enhancing the metastatic potential of the tumors.

PubMed Disclaimer

Cited by

Heparanase contributes to pancreatic carcinoma progression through insulin-dependent glucose uptake.
Abecassis A, Hermano E, Yifrach A, Popovtzer A, Meirovitz A, Elkin M. Abecassis A, et al. Front Cell Dev Biol. 2023 Nov 22;11:1287084. doi: 10.3389/fcell.2023.1287084. eCollection 2023. Front Cell Dev Biol. 2023. PMID: 38078007 Free PMC article.
Mammalian heparanase: what is the message?
Vreys V, David G. Vreys V, et al. J Cell Mol Med. 2007 May-Jun;11(3):427-52. doi: 10.1111/j.1582-4934.2007.00039.x. J Cell Mol Med. 2007. PMID: 17635638 Free PMC article. Review.
Synergistic effects of L- and P-selectin in facilitating tumor metastasis can involve non-mucin ligands and implicate leukocytes as enhancers of metastasis.
Borsig L, Wong R, Hynes RO, Varki NM, Varki A. Borsig L, et al. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2193-8. doi: 10.1073/pnas.261704098. Proc Natl Acad Sci U S A. 2002. PMID: 11854515 Free PMC article.
Biochemical characterization of the active heterodimer form of human heparanase (Hpa1) protein expressed in insect cells.
McKenzie E, Young K, Hircock M, Bennett J, Bhaman M, Felix R, Turner P, Stamps A, McMillan D, Saville G, Ng S, Mason S, Snell D, Schofield D, Gong H, Townsend R, Gallagher J, Page M, Parekh R, Stubberfield C. McKenzie E, et al. Biochem J. 2003 Jul 15;373(Pt 2):423-35. doi: 10.1042/BJ20030318. Biochem J. 2003. PMID: 12713442 Free PMC article.
Exosomes are the Driving Force in Preparing the Soil for the Metastatic Seeds: Lessons from the Prostate Cancer.
Saber SH, Ali HEA, Gaballa R, Gaballah M, Ali HI, Zerfaoui M, Abd Elmageed ZY. Saber SH, et al. Cells. 2020 Feb 28;9(3):564. doi: 10.3390/cells9030564. Cells. 2020. PMID: 32121073 Free PMC article. Review.

Heparanase expression in primary and metastatic pancreatic cancer - PubMed (original) (raw)