New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants - PubMed (original) (raw)

New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants

R Wagner et al. J Bacteriol. 2001 Jul.

Abstract

The FCY2 gene of Saccharomyces cerevisiae encodes a purine-cytosine permease (PCP) that mediates the active transport of purines and cytosine. A structure-function model for this PCP has been recently proposed. In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function.

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Figures

FIG. 1

FIG. 1

Positions of selected amino acid substitutions in the PCP model proposed by Ferreira et al. (7).

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