Functional analysis of secreted and transmembrane proteins critical to mouse development - PubMed (original) (raw)

K I Pinson, O G Kelly, J Brennan, J Zupicich, P Scherz, P A Leighton, L V Goodrich, X Lu, B J Avery, P Tate, K Dill, E Pangilinan, P Wakenight, M Tessier-Lavigne, W C Skarnes

Affiliations

• PMID: 11431694
• DOI: 10.1038/90074

Functional analysis of secreted and transmembrane proteins critical to mouse development

K J Mitchell et al. Nat Genet. 2001 Jul.

Abstract

We describe the successful application of a modified gene-trap approach, the secretory trap, to systematically analyze the functions in vivo of large numbers of genes encoding secreted and membrane proteins. Secretory-trap insertions in embryonic stem cells can be transmitted to the germ line of mice with high efficiency and effectively mutate the target gene. Of 60 insertions analyzed in mice, one-third cause recessive lethal phenotypes affecting various stages of embryonic and postnatal development. Thus, secretory-trap mutagenesis can be used for a genome-wide functional analysis of cell signaling pathways that are critical for normal mammalian development and physiology.

PubMed Disclaimer

Comment in

• Mouse mutagenesis on target.
  Jackson IJ. Jackson IJ. Nat Genet. 2001 Jul;28(3):198-200. doi: 10.1038/90017. Nat Genet. 2001. PMID: 11431680

Similar articles

• High-throughput trapping of secretory pathway genes in mouse embryonic stem cells.
  De-Zolt S, Schnütgen F, Seisenberger C, Hansen J, Hollatz M, Floss T, Ruiz P, Wurst W, von Melchner H. De-Zolt S, et al. Nucleic Acids Res. 2006 Feb 13;34(3):e25. doi: 10.1093/nar/gnj026. Nucleic Acids Res. 2006. PMID: 16478711 Free PMC article.
• Mouse gene trap approach: identification of novel genes and characterization of their biological functions.
  Kitajima K, Takeuchi T. Kitajima K, et al. Biochem Cell Biol. 1998;76(6):1029-37. Biochem Cell Biol. 1998. PMID: 10392714 Review.
• Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.
  Schnütgen F, De-Zolt S, Van Sloun P, Hollatz M, Floss T, Hansen J, Altschmied J, Seisenberger C, Ghyselinck NB, Ruiz P, Chambon P, Wurst W, von Melchner H. Schnütgen F, et al. Proc Natl Acad Sci U S A. 2005 May 17;102(20):7221-6. doi: 10.1073/pnas.0502273102. Epub 2005 May 3. Proc Natl Acad Sci U S A. 2005. PMID: 15870191 Free PMC article.
• Gene trap mutagenesis in embryonic stem cells.
  Chen WV, Soriano P. Chen WV, et al. Methods Enzymol. 2003;365:367-86. Methods Enzymol. 2003. PMID: 14696359 No abstract available.
• Insertional mutagenesis in mice: new perspectives and tools.
  Carlson CM, Largaespada DA. Carlson CM, et al. Nat Rev Genet. 2005 Jul;6(7):568-80. doi: 10.1038/nrg1638. Nat Rev Genet. 2005. PMID: 15995698 Review.

Cited by

• Organogenetic transcriptomes of the Drosophila embryo at single cell resolution.
  Peng D, Jackson D, Palicha B, Kernfeld E, Laughner N, Shoemaker A, Celniker SE, Loganathan R, Cahan P, Andrew DJ. Peng D, et al. Development. 2024 Jan 15;151(2):dev202097. doi: 10.1242/dev.202097. Epub 2024 Jan 18. Development. 2024. PMID: 38174902 Free PMC article.
• Left ventricular hypertrophy and metabolic resetting in the Notch3-deficient adult mouse heart.
  Del Gaudio F, Liu D, Andaloussi Mäe M, Braune EB, Hansson EM, Wang QD, Betsholtz C, Lendahl U. Del Gaudio F, et al. Sci Rep. 2023 Sep 12;13(1):15022. doi: 10.1038/s41598-023-42010-7. Sci Rep. 2023. PMID: 37699967 Free PMC article.
• Selective involvement of UGGT variant: UGGT2 in protecting mouse embryonic fibroblasts from saturated lipid-induced ER stress.
  Hung HH, Nagatsuka Y, Soldà T, Kodali VK, Iwabuchi K, Kamiguchi H, Kano K, Matsuo I, Ikeda K, Kaufman RJ, Molinari M, Greimel P, Hirabayashi Y. Hung HH, et al. Proc Natl Acad Sci U S A. 2022 Dec 20;119(51):e2214957119. doi: 10.1073/pnas.2214957119. Epub 2022 Dec 12. Proc Natl Acad Sci U S A. 2022. PMID: 36508673 Free PMC article.
• A genetic model for in vivo proximity labelling of the mammalian secretome.
  Yang R, Meyer AS, Droujinine IA, Udeshi ND, Hu Y, Guo J, McMahon JA, Carey DK, Xu C, Fang Q, Sha J, Qin S, Rocco D, Wohlschlegel J, Ting AY, Carr SA, Perrimon N, McMahon AP. Yang R, et al. Open Biol. 2022 Aug;12(8):220149. doi: 10.1098/rsob.220149. Epub 2022 Aug 10. Open Biol. 2022. PMID: 35946312 Free PMC article.
• Specification of CNS macrophage subsets occurs postnatally in defined niches.
  Masuda T, Amann L, Monaco G, Sankowski R, Staszewski O, Krueger M, Del Gaudio F, He L, Paterson N, Nent E, Fernández-Klett F, Yamasaki A, Frosch M, Fliegauf M, Bosch LFP, Ulupinar H, Hagemeyer N, Schreiner D, Dorrier C, Tsuda M, Grothe C, Joutel A, Daneman R, Betsholtz C, Lendahl U, Knobeloch KP, Lämmermann T, Priller J, Kierdorf K, Prinz M. Masuda T, et al. Nature. 2022 Apr;604(7907):740-748. doi: 10.1038/s41586-022-04596-2. Epub 2022 Apr 20. Nature. 2022. PMID: 35444273

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • H1 Connect
  • The Lens - Patent Citations

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)