From hematopoiesis to neuropoiesis: evidence of overlapping genetic programs - PubMed (original) (raw)
From hematopoiesis to neuropoiesis: evidence of overlapping genetic programs
A V Terskikh et al. Proc Natl Acad Sci U S A. 2001.
Abstract
It is reasonable to propose that gene expression profiles of purified stem cells could give clues for the molecular mechanisms of stem cell behavior. We took advantage of cDNA subtraction to identify a set of genes selectively expressed in mouse adult hematopoietic stem cells (HSC) as opposed to bone marrow (BM). Analysis of HSC-enriched genes revealed several key regulatory gene candidates, including two novel seven transmembrane (7TM) receptors. Furthermore, by using cDNA microarray techniques we found a large set of HSC-enriched genes that are expressed in mouse neurospheres (a population greatly enriched for neural progenitor cells), but not present in terminally differentiated neural cells. In situ hybridization demonstrated that many of them, including one HSC-enriched 7TM receptor, were selectively expressed in the germinal zones of fetal and adult brain, the regions harboring mouse neural stem cells. We propose that at least some of the transcripts that are selectively and commonly expressed in two or more types of stem cells define a functionally conserved group of genes evolved to participate in basic stem cell functions, including stem cell self-renewal.
Figures
Figure 1
Analysis of genes found in the HB library. (A) Categorization by sequence type. (B) Functional clustering of known (or highly homologous) genes. (C) Sequence composition of 223 nonredundant contigs.
Figure 2
The reverse transcription–PCR analysis of selected genes found in the HB library. Each gene was tested by using three independently prepared samples of double-sorted HSC and BM.
Figure 3
In situ hybridization analysis of HSC-NS common-specific genes. (A_–_F) Bright field images: E13 whole embryo, E17 head, P1 brain, and adult brain. (G_–_J) Emulsion-dipped autoradiograms:G and I, brains; H, liver;J, cranial nerve 5. (Scale bar, 250 μm.)
Figure 4
Schematic representation of gene expression in different stem cell populations.
Comment in
- Chipping away at stem cells.
Chu VT, Gage FH. Chu VT, et al. Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7652-3. doi: 10.1073/pnas.141244198. Proc Natl Acad Sci U S A. 2001. PMID: 11438717 Free PMC article. No abstract available.
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