Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo-beta-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae - PubMed (original) (raw)

Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo-beta-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae

J J Yan et al. Antimicrob Agents Chemother. 2001 Aug.

Abstract

A multidrug-resistant plasmid encoding TEM-1, SHV-12, and a variant of IMP-2 metallo-beta-lactamase, designated IMP-8, was identified from a clinical isolate of Klebsiella pneumoniae. There are four nucleotide differences between bla(IMP-2) and bla(IMP-8), resulting in two amino acid differences. bla(IMP-8) was also found to be carried by an integron-borne gene cassette similar to the bla(IMP-2) cassette.

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Figures

FIG. 1

FIG. 1

Nucleotide sequence of the _bla_IMP-8 gene and flanking regions. The deduced amino acid sequence of _bla_IMP-8 is indicated in the single-letter code below the nucleotide sequence of _bla_IMP-8. The start codons of _intII, bla_IMP-8, and aac(6′)Ib are indicated by horizontal arrows, and the stop codon of _bla_IMP-8 is indicated by three asterisks. The nucleotides and amino acids that differ between _bla_IMP-8 and _bla_IMP-2 are marked by bold letters, and the nucleotides and amino acids for _bla_IMP-2 and IMP-2 that differ from the sequences for _bla_IMP-8 and IMP-8 are shown above and below the sequences. The _bla_IMP-8 cassette boundaries are indicated by vertical arrows. The conserved core and inverse core sites located at the _bla_IMP-8 cassette boundaries are boxed.

FIG. 2

FIG. 2

Results of isoelectric focusing analysis. Lane 1, K. pneumoniae KPO787; lane 2, E. coli C600(pEKPO787); lane 3, E. coli HB101(pEKPO787D1); lane 4, K. pneumoniae KPT986, a clinical isolate known to produce TEM-1 (pI 5.4), SHV-1 (pI 7.6), and SHV-12 (pI 8.2) (18). The numbers on the right are pIs.

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