Identification of DNA-protein interactions in the 5' flanking and 5' untranslated regions of the human multidrug resistance protein (MRP1) gene: evaluation of a putative antioxidant response element/AP-1 binding site - PubMed (original) (raw)
. 2001 Jul 27;285(4):981-90.
doi: 10.1006/bbrc.2001.5262.
Affiliations
- PMID: 11467849
- DOI: 10.1006/bbrc.2001.5262
Identification of DNA-protein interactions in the 5' flanking and 5' untranslated regions of the human multidrug resistance protein (MRP1) gene: evaluation of a putative antioxidant response element/AP-1 binding site
E U Kurz et al. Biochem Biophys Res Commun. 2001.
Abstract
Overexpression of the multidrug resistance protein, MRP1, confers resistance to multiple natural product-type chemotherapeutics. MRP1 amplification is observed in some multidrug-resistant cell lines, while in others, increased transcription occurs in the absence of gene amplification. To investigate mechanisms influencing MRP1 transcription, three small cell lung cancer cell lines were examined: drug sensitive H69 cells with two apparently normal MRP1 alleles, highly resistant H69AR cells in which MRP1 is amplified and low level resistant H69PR cells that contain only one MRP1 allele. Deoxyribonuclease I footprinting and gel mobility shift assays were undertaken using nuclear extracts from the three cell lines and a 1 kb region encompassing the 5' flanking region of MRP1. Thirteen protein binding sites were identified of which six were sequence specific. Differences in levels of protein binding occurred with a putative antioxidant response element (ARE)/AP-1 binding site at -511 to -477. Levels of protein binding to this site were 2.5- to 3.0-fold higher in H69AR nuclear extracts versus extracts from H69 or H69PR cells. The AP-1 sequence is required for binding and c-Jun and JunD were identified as components of the protein complex. The ARE/AP-1 element functioned as a transcriptional enhancer but did not mediate induction of a luciferase reporter gene upon beta-naphthoflavone treatment.
Copyright 2001 Academic Press.
Similar articles
- Expression of human MRP6, a homologue of the multidrug resistance protein gene MRP1, in tissues and cancer cells.
Kool M, van der Linden M, de Haas M, Baas F, Borst P. Kool M, et al. Cancer Res. 1999 Jan 1;59(1):175-82. Cancer Res. 1999. PMID: 9892204 - Nrf2 pathway regulates multidrug-resistance-associated protein 1 in small cell lung cancer.
Ji L, Li H, Gao P, Shang G, Zhang DD, Zhang N, Jiang T. Ji L, et al. PLoS One. 2013 May 7;8(5):e63404. doi: 10.1371/journal.pone.0063404. Print 2013. PLoS One. 2013. PMID: 23667609 Free PMC article. - The human multidrug resistance protein 2 gene: functional characterization of the 5'-flanking region and expression in hepatic cells.
Tanaka T, Uchiumi T, Hinoshita E, Inokuchi A, Toh S, Wada M, Takano H, Kohno K, Kuwano M. Tanaka T, et al. Hepatology. 1999 Dec;30(6):1507-12. doi: 10.1002/hep.510300617. Hepatology. 1999. PMID: 10573531 - Identification of a decidua-specific enhancer on the human prolactin gene with two critical activator protein 1 (AP-1) binding sites.
Watanabe K, Kessler CA, Bachurski CJ, Kanda Y, Richardson BD, Stanek J, Handwerger S, Brar AK. Watanabe K, et al. Mol Endocrinol. 2001 Apr;15(4):638-53. doi: 10.1210/mend.15.4.0623. Mol Endocrinol. 2001. PMID: 11266514 - The human multidrug resistance-associated protein (MRP) gene family: from biological function to drug molecular design.
Ishikawa T, Kuo MT, Furuta K, Suzuki M. Ishikawa T, et al. Clin Chem Lab Med. 2000 Sep;38(9):893-7. doi: 10.1515/CCLM.2000.130. Clin Chem Lab Med. 2000. PMID: 11097346 Review.
Cited by
- Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine.
Valera ET, de Freitas Cortez MA, de Paula Queiroz RG, de Oliveira FM, Brassesco MS, Jabado N, Faury D, Bobola MS, Machado HR, Scrideli CA, Tone LG. Valera ET, et al. Childs Nerv Syst. 2009 Jan;25(1):39-45. doi: 10.1007/s00381-008-0740-3. Epub 2008 Oct 23. Childs Nerv Syst. 2009. PMID: 18946672 - Notch1 regulates the expression of the multidrug resistance gene ABCC1/MRP1 in cultured cancer cells.
Cho S, Lu M, He X, Ee PL, Bhat U, Schneider E, Miele L, Beck WT. Cho S, et al. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20778-83. doi: 10.1073/pnas.1019452108. Epub 2011 Dec 5. Proc Natl Acad Sci U S A. 2011. PMID: 22143792 Free PMC article. - Collagen/β1 integrin signaling up-regulates the ABCC1/MRP-1 transporter in an ERK/MAPK-dependent manner.
El Azreq MA, Naci D, Aoudjit F. El Azreq MA, et al. Mol Biol Cell. 2012 Sep;23(17):3473-84. doi: 10.1091/mbc.E12-02-0132. Epub 2012 Jul 11. Mol Biol Cell. 2012. PMID: 22787275 Free PMC article. - Hypoxia, oxidative stress, and the interplay of HIFs and NRF2 signaling in cancer.
Bae T, Hallis SP, Kwak MK. Bae T, et al. Exp Mol Med. 2024 Mar;56(3):501-514. doi: 10.1038/s12276-024-01180-8. Epub 2024 Mar 1. Exp Mol Med. 2024. PMID: 38424190 Free PMC article. Review. - Identification of novel NRF2-regulated genes by ChIP-Seq: influence on retinoid X receptor alpha.
Chorley BN, Campbell MR, Wang X, Karaca M, Sambandan D, Bangura F, Xue P, Pi J, Kleeberger SR, Bell DA. Chorley BN, et al. Nucleic Acids Res. 2012 Aug;40(15):7416-29. doi: 10.1093/nar/gks409. Epub 2012 May 11. Nucleic Acids Res. 2012. PMID: 22581777 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous