Cytokine-induced activation of nuclear factor-kappa B is inhibited by hydrogen peroxide through oxidative inactivation of IkappaB kinase - PubMed (original) (raw)
. 2001 Sep 21;276(38):35693-700.
doi: 10.1074/jbc.M104321200. Epub 2001 Jul 30.
Affiliations
- PMID: 11479295
- DOI: 10.1074/jbc.M104321200
Free article
Cytokine-induced activation of nuclear factor-kappa B is inhibited by hydrogen peroxide through oxidative inactivation of IkappaB kinase
S H Korn et al. J Biol Chem. 2001.
Free article
Abstract
Rapid activation of the IkappaB kinase (IKK) complex is considered an obligatory step in the activation of nuclear factor-kappaB (NF-kappaB) in response to diverse stimuli. Since oxidants have been implicated in the regulation of NF-kappaB, the focus of the present study was the activation of IKK by tumor necrosis factor alpha (TNFalpha) in the presence or absence of hydrogen peroxide (H(2)O(2)). Exposure of mouse alveolar epithelial cells to H(2)O(2) was not sufficient to activate IKK, degrade IkappaBalpha, or activate NF-kappaB. In contrast, TNFalpha induced IKK activity rapidly and transiently resulting in IkappaBalpha degradation and NF-kappaB activation. Importantly, in the presence of H(2)O(2), the ability of TNFalpha to induce IKK activity was markedly decreased and resulted in prevention of IkappaBalpha degradation and NF-kappaB activation. Neither tyrosine kinases nor phosphatidylinositol 3-kinases, known regulators of NF-kappaB by oxidants, were involved in IKK inhibition by H(2)O(2). Direct addition of H(2)O(2) to the immunoprecipitated IKK complex inhibited enzyme activity. Inhibition of IKK activity by H(2)O(2) was associated with direct oxidation of cysteine residues present in the IKK complex and occurred only in enzymatically active IKK. In contrast to previously published observations, our findings demonstrate that the oxidant H(2)O(2) reduces NF-kappaB activation by inhibiting activated IKK activity.
Similar articles
- Hydrogen peroxide-mediated inhibition of lipopolysaccharide-stimulated inhibitory kappa B kinase activity in rat aortic smooth muscle cells.
Torrie LJ, MacKenzie CJ, Paul A, Plevin R. Torrie LJ, et al. Br J Pharmacol. 2001 Sep;134(2):393-401. doi: 10.1038/sj.bjp.0704259. Br J Pharmacol. 2001. PMID: 11564658 Free PMC article. - Hydrogen peroxide has opposing effects on IKK activity and IkappaBalpha breakdown in airway epithelial cells.
Jaspers I, Zhang W, Fraser A, Samet JM, Reed W. Jaspers I, et al. Am J Respir Cell Mol Biol. 2001 Jun;24(6):769-77. doi: 10.1165/ajrcmb.24.6.4344. Am J Respir Cell Mol Biol. 2001. PMID: 11415944 - The non-provitamin A carotenoid, lutein, inhibits NF-kappaB-dependent gene expression through redox-based regulation of the phosphatidylinositol 3-kinase/PTEN/Akt and NF-kappaB-inducing kinase pathways: role of H(2)O(2) in NF-kappaB activation.
Kim JH, Na HJ, Kim CK, Kim JY, Ha KS, Lee H, Chung HT, Kwon HJ, Kwon YG, Kim YM. Kim JH, et al. Free Radic Biol Med. 2008 Sep 15;45(6):885-96. doi: 10.1016/j.freeradbiomed.2008.06.019. Epub 2008 Jun 27. Free Radic Biol Med. 2008. PMID: 18620044 - Regulation and function of IKK and IKK-related kinases.
Häcker H, Karin M. Häcker H, et al. Sci STKE. 2006 Oct 17;2006(357):re13. doi: 10.1126/stke.3572006re13. Sci STKE. 2006. PMID: 17047224 Review.
Cited by
- The Role of Oxidative Stress in Hypomagnetic Field Effects.
Tian L, Luo Y, Ren J, Zhao C. Tian L, et al. Antioxidants (Basel). 2024 Aug 21;13(8):1017. doi: 10.3390/antiox13081017. Antioxidants (Basel). 2024. PMID: 39199261 Free PMC article. Review. - Redox signaling and skeletal muscle adaptation during aerobic exercise.
Zhou Y, Zhang X, Baker JS, Davison GW, Yan X. Zhou Y, et al. iScience. 2024 Mar 29;27(5):109643. doi: 10.1016/j.isci.2024.109643. eCollection 2024 May 17. iScience. 2024. PMID: 38650987 Free PMC article. Review. - The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control.
Yamamoto S, Ogasawara N, Mitsuhashi Y, Takano K, Yokota SI. Yamamoto S, et al. Sci Rep. 2024 Jan 29;14(1):2354. doi: 10.1038/s41598-024-52582-7. Sci Rep. 2024. PMID: 38287119 Free PMC article. - Mitochondrial Impairment: A Link for Inflammatory Responses Activation in the Cardiorenal Syndrome Type 4.
Amador-Martínez I, Aparicio-Trejo OE, Bernabe-Yepes B, Aranda-Rivera AK, Cruz-Gregorio A, Sánchez-Lozada LG, Pedraza-Chaverri J, Tapia E. Amador-Martínez I, et al. Int J Mol Sci. 2023 Nov 1;24(21):15875. doi: 10.3390/ijms242115875. Int J Mol Sci. 2023. PMID: 37958859 Free PMC article. Review. - An Adverse Outcome Pathway Network for Chemically Induced Oxidative Stress Leading to (Non)genotoxic Carcinogenesis.
Veltman CHJ, Pennings JLA, van de Water B, Luijten M. Veltman CHJ, et al. Chem Res Toxicol. 2023 Jun 19;36(6):805-817. doi: 10.1021/acs.chemrestox.2c00396. Epub 2023 May 8. Chem Res Toxicol. 2023. PMID: 37156502 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous