Interaction of hemoglobin with enterobacterial lipopolysaccharide and lipid A. Physicochemical characterization and biological activity - PubMed (original) (raw)
Interaction of hemoglobin with enterobacterial lipopolysaccharide and lipid A. Physicochemical characterization and biological activity
G Jürgens et al. Eur J Biochem. 2001 Aug.
Free article
Abstract
The interaction of hemoglobin (Hb) with endotoxins [i.e. with enterobacterial deep rough mutant lipopolysaccharide (LPS) Re and the "endotoxic principle" of LPS, lipid A] was investigated using a variety of physical techniques and with two biological assays, tumor necrosis factor (TNF)-alpha induction in human mononuclear cells and the Limulus amebocyte lysate (LAL) assay. Fourier-transform IR-spectroscopic experiments indicate nonelectrostatic binding to the hydrophobic moiety with a slight rigidification of the lipid A acyl chains, and an increase in the inclination of the lipid A backbone with respect to the membrane surface from 35 degrees to more than 40 degrees due to Hb binding, but no change of the predominantly alpha-helical secondary structures of Hb due to LPS binding. From isothermal titration calorimetry, the molar [Hb] : [endotoxin] binding ratio lies between 1 : 3 and 1 : 5 molar. Synchrotron radiation X-ray diffraction measurements indicate a reorientation of the lipid A aggregates from one cubic structure to another, the final structure belonging to space group Q224. The LPS-induced TNF-alpha production of mononuclear cells is enhanced by Hb, whereas in the LAL assay an LPS concentration-dependent increase or decrease was observed. Although a detailed mechanism of action cannot be given, the enhancement of LPS bioactivity can be understood in the light of the previously presented conformational concept; Hb induces an increase in the conical shape of the lipid A moiety of LPS, higher cross-section of the hydrophobic than the hydrophilic part, and of the inclination angle of the diglucosamine backbone with respect to the direction of the acyl chains.
Similar articles
- Cross-linked hemoglobin converts endotoxically inactive pentaacyl endotoxins into a physiologically active conformation.
Brandenburg K, Garidel P, Andra J, Jürgens G, Müller M, Blume A, Koch MH, Levin J. Brandenburg K, et al. J Biol Chem. 2003 Nov 28;278(48):47660-9. doi: 10.1074/jbc.M304743200. Epub 2003 Sep 17. J Biol Chem. 2003. PMID: 13679376 - Biophysical characterization of the interaction of Limulus polyphemus endotoxin neutralizing protein with lipopolysaccharide.
Andrä J, Garidel P, Majerle A, Jerala R, Ridge R, Paus E, Novitsky T, Koch MH, Brandenburg K. Andrä J, et al. Eur J Biochem. 2004 May;271(10):2037-46. doi: 10.1111/j.1432-1033.2004.04134.x. Eur J Biochem. 2004. PMID: 15128313 - Physicochemical characterization of the endotoxins from Coxiella burnetii strain Priscilla in relation to their bioactivities.
Toman R, Garidel P, Andrä J, Slaba K, Hussein A, Koch MH, Brandenburg K. Toman R, et al. BMC Biochem. 2004 Jan 12;5:1. doi: 10.1186/1471-2091-5-1. BMC Biochem. 2004. PMID: 14715092 Free PMC article. - The expression of endotoxic activity in the Limulus test as compared to cytokine production in immune cells.
Brandenburg K, Howe J, Gutsman T, Garidel P. Brandenburg K, et al. Curr Med Chem. 2009;16(21):2653-60. doi: 10.2174/092986709788682001. Curr Med Chem. 2009. PMID: 19601802 Review. - A case for an endotoxic conformation.
Seydel U, Brandenburg K, Rietschel ET. Seydel U, et al. Prog Clin Biol Res. 1994;388:17-30. Prog Clin Biol Res. 1994. PMID: 7831357 Review.
Cited by
- From In Vitro Promise to In Vivo Reality: An Instructive Account of Infection Model Evaluation of Antimicrobial Peptides.
Carrera-Aubesart A, Li J, Contreras E, Bello-Madruga R, Torrent M, Andreu D. Carrera-Aubesart A, et al. Int J Mol Sci. 2024 Sep 10;25(18):9773. doi: 10.3390/ijms25189773. Int J Mol Sci. 2024. PMID: 39337261 Free PMC article. - In Vivo Evaluation of ECP Peptide Analogues for the Treatment of Acinetobacter baumannii Infection.
Li J, Prats-Ejarque G, Torrent M, Andreu D, Brandenburg K, Fernández-Millán P, Boix E. Li J, et al. Biomedicines. 2022 Feb 5;10(2):386. doi: 10.3390/biomedicines10020386. Biomedicines. 2022. PMID: 35203595 Free PMC article. - Optimized Triton X-114 assisted lipopolysaccharide (LPS) removal method reveals the immunomodulatory effect of food proteins.
Teodorowicz M, Perdijk O, Verhoek I, Govers C, Savelkoul HF, Tang Y, Wichers H, Broersen K. Teodorowicz M, et al. PLoS One. 2017 Mar 29;12(3):e0173778. doi: 10.1371/journal.pone.0173778. eCollection 2017. PLoS One. 2017. PMID: 28355240 Free PMC article. - Methemoglobin-induced signaling and chemokine responses in human alveolar epithelial cells.
Mumby S, Ramakrishnan L, Evans TW, Griffiths MJ, Quinlan GJ. Mumby S, et al. Am J Physiol Lung Cell Mol Physiol. 2014 Jan 1;306(1):L88-100. doi: 10.1152/ajplung.00066.2013. Epub 2013 Oct 18. Am J Physiol Lung Cell Mol Physiol. 2014. PMID: 24142518 Free PMC article. - Delineation of lipopolysaccharide (LPS)-binding sites on hemoglobin: from in silico predictions to biophysical characterization.
Bahl N, Du R, Winarsih I, Ho B, Tucker-Kellogg L, Tidor B, Ding JL. Bahl N, et al. J Biol Chem. 2011 Oct 28;286(43):37793-803. doi: 10.1074/jbc.M111.245472. Epub 2011 Sep 6. J Biol Chem. 2011. PMID: 21900232 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources