Inhibition of connexin43 gap junctional intercellular communication by TPA requires ERK activation - PubMed (original) (raw)
Inhibition of connexin43 gap junctional intercellular communication by TPA requires ERK activation
R J Ruch et al. J Cell Biochem. 2001.
Abstract
The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), is a potent inhibitor of gap junctional intercellular communication (GJIC). This inhibition requires activation of protein kinase C (PKC), but the events downstream of this kinase are not known. Since PKC can activate extracellular signal regulated kinases (ERKs) and these also downregulate GJIC, we hypothesized that the inhibition of GJIC by TPA involved ERKs. TPA treatment (10 ng/ml for 30 min) of WB-F344 rat liver epithelial cells strongly activated p42 and p44 ERK-1 and -2, blocked gap junction-mediated fluorescent dye-coupling, and induced connexin43 hyperphosphorylation and gap junction internalization. These effects were completely prevented by inhibitors of PKC (bis-indolylmaleimide I; 2 microM) and ERK activation (U-0126; 10 microM). These data suggest that ERKs are activated by PKC in response to TPA treatment and are downstream mediators of the gap junction effects of the phorbol ester.
Copyright 2001 Wiley-Liss, Inc.
Similar articles
- Recovery of gap junctional intercellular communication after phorbol ester treatment requires proteasomal degradation of protein kinase C.
Leithe E, Cruciani V, Sanner T, Mikalsen SO, Rivedal E. Leithe E, et al. Carcinogenesis. 2003 Jul;24(7):1239-45. doi: 10.1093/carcin/bgg066. Epub 2003 May 9. Carcinogenesis. 2003. PMID: 12807762 - Altered gap junction activity in cardiovascular tissues of diabetes.
Inoguchi T, Yu HY, Imamura M, Kakimoto M, Kuroki T, Maruyama T, Nawata H. Inoguchi T, et al. Med Electron Microsc. 2001 Jun;34(2):86-91. doi: 10.1007/s007950170002. Med Electron Microsc. 2001. PMID: 11685657 Review.
Cited by
- Salidroside Protects against Cadmium-Induced Hepatotoxicity in Rats via GJIC and MAPK Pathways.
Zou H, Liu X, Han T, Hu D, Wang Y, Yuan Y, Gu J, Bian J, Zhu J, Liu ZP. Zou H, et al. PLoS One. 2015 Jun 12;10(6):e0129788. doi: 10.1371/journal.pone.0129788. eCollection 2015. PLoS One. 2015. PMID: 26070151 Free PMC article. - Inhibition of gap junctional Intercellular communication in WB-F344 rat liver epithelial cells by triphenyltin chloride through MAPK and PI3-kinase pathways.
Lee CH, Chen IH, Lee CR, Chi CH, Tsai MC, Tsai JL, Lin HF. Lee CH, et al. J Occup Med Toxicol. 2010 Jun 30;5:17. doi: 10.1186/1745-6673-5-17. J Occup Med Toxicol. 2010. PMID: 20591183 Free PMC article. - Activation of PDGFRA signaling contributes to filamin C-related arrhythmogenic cardiomyopathy.
Chen SN, Lam CK, Wan YW, Gao S, Malak OA, Zhao SR, Lombardi R, Ambardekar AV, Bristow MR, Cleveland J, Gigli M, Sinagra G, Graw S, Taylor MRG, Wu JC, Mestroni L. Chen SN, et al. Sci Adv. 2022 Feb 25;8(8):eabk0052. doi: 10.1126/sciadv.abk0052. Epub 2022 Feb 23. Sci Adv. 2022. PMID: 35196083 Free PMC article. - Adrenocortical Gap Junctions and Their Functions.
Bell CL, Murray SA. Bell CL, et al. Front Endocrinol (Lausanne). 2016 Jun 29;7:82. doi: 10.3389/fendo.2016.00082. eCollection 2016. Front Endocrinol (Lausanne). 2016. PMID: 27445985 Free PMC article. Review. - Panx3 links body mass index and tumorigenesis in a genetically heterogeneous mouse model of carcinogen-induced cancer.
Halliwill KD, Quigley DA, Kang HC, Del Rosario R, Ginzinger D, Balmain A. Halliwill KD, et al. Genome Med. 2016 Aug 9;8(1):83. doi: 10.1186/s13073-016-0334-8. Genome Med. 2016. PMID: 27506198 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous