Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster - PubMed (original) (raw)
. 2001 Aug 17;293(5533):1281-5.
doi: 10.1126/science.1061500.
Affiliations
- PMID: 11509720
- DOI: 10.1126/science.1061500
Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster
H Dobbek et al. Science. 2001.
Abstract
The homodimeric nickel-containing CO dehydrogenase from the anaerobic bacterium Carboxydothermus hydrogenoformans catalyzes the oxidation of CO to CO2. A crystal structure of the reduced enzyme has been solved at 1.6 angstrom resolution. This structure represents the prototype for Ni-containing CO dehydrogenases from anaerobic bacteria and archaea. It contains five metal clusters of which clusters B, B', and a subunit-bridging, surface-exposed cluster D are cubane-type [4Fe-4S] clusters. The active-site clusters C and C' are novel, asymmetric [Ni-4Fe-5S] clusters. Their integral Ni ion, which is the likely site of CO oxidation, is coordinated by four sulfur ligands with square planar geometry.
Comment in
- Enzymology. Nickel to the fore.
Thauer RK. Thauer RK. Science. 2001 Aug 17;293(5533):1264-5. doi: 10.1126/science.1064049. Science. 2001. PMID: 11509713 No abstract available.
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