Preferential transduction of neurons by canine adenovirus vectors and their efficient retrograde transport in vivo - PubMed (original) (raw)
. 2001 Oct;15(12):2283-5.
doi: 10.1096/fj.01-0321fje. Epub 2001 Aug 17.
Affiliations
- PMID: 11511531
- DOI: 10.1096/fj.01-0321fje
Preferential transduction of neurons by canine adenovirus vectors and their efficient retrograde transport in vivo
C Soudais et al. FASEB J. 2001 Oct.
Abstract
In the central nervous system (CNS), there are innate obstacles to the modification of neurons: their relative low abundance versus glia and oligodendrocytes, the inaccessibility of certain target populations, and the volume one can inject safely. Our aim in this study was to characterize the in vivo efficacy of a novel viral vector derived from a canine adenovirus (CAV-2). Here we show that CAV-2 preferentially transduced i) rat olfactory sensory neurons; ii) rodent CNS neurons in vitro and in vivo; and, more clinically relevant, iii) neurons in organotypic slices of human cortical brain. CAV-2 also showed a high disposition for retrograde axonal transport in vivo. We examined the molecular basis of neuronal targeting by CAV-2 and suggest that due to CAR (coxsackie adenovirus receptor) expression on neuronal cells-and not oligodendrocytes, glia, myofibers, and nasal epithelial cells-CAV-2 vectors transduced neurons preferentially in these diverse tissues.
Similar articles
- Long-term in vivo transduction of neurons throughout the rat CNS using novel helper-dependent CAV-2 vectors.
Soudais C, Skander N, Kremer EJ. Soudais C, et al. FASEB J. 2004 Feb;18(2):391-3. doi: 10.1096/fj.03-0438fje. Epub 2003 Dec 19. FASEB J. 2004. PMID: 14688208 - Study of adenovirus and CAR axonal transport in primary neurons.
Zussy C, Salinas S. Zussy C, et al. Methods Mol Biol. 2014;1089:71-8. doi: 10.1007/978-1-62703-679-5_5. Methods Mol Biol. 2014. PMID: 24132478 - Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model.
Simão D, Pinto C, Fernandes P, Peddie CJ, Piersanti S, Collinson LM, Salinas S, Saggio I, Schiavo G, Kremer EJ, Brito C, Alves PM. Simão D, et al. Gene Ther. 2016 Jan;23(1):86-94. doi: 10.1038/gt.2015.75. Epub 2015 Aug 20. Gene Ther. 2016. PMID: 26181626 Free PMC article. - CAV-2--why a canine virus is a neurobiologist's best friend.
Junyent F, Kremer EJ. Junyent F, et al. Curr Opin Pharmacol. 2015 Oct;24:86-93. doi: 10.1016/j.coph.2015.08.004. Epub 2015 Aug 25. Curr Opin Pharmacol. 2015. PMID: 26298516 Review. - The conundrum between immunological memory to adenovirus and their use as vectors in clinical gene therapy.
Perreau M, Kremer EJ. Perreau M, et al. Mol Biotechnol. 2006 Oct;34(2):247-56. doi: 10.1385/MB:34:2:247. Mol Biotechnol. 2006. PMID: 17172670 Review.
Cited by
- The Projection-Specific Noradrenergic Modulation of Perseverative Spatial Behavior in Adult Male Rats.
Kabanova A, Fedorov L, Eschenko O. Kabanova A, et al. eNeuro. 2024 Aug 19;11(8):ENEURO.0063-24.2024. doi: 10.1523/ENEURO.0063-24.2024. Print 2024 Aug. eNeuro. 2024. PMID: 39160074 Free PMC article. - Integrin-Targeting Strategies for Adenovirus Gene Therapy.
Nemerow GR. Nemerow GR. Viruses. 2024 May 13;16(5):770. doi: 10.3390/v16050770. Viruses. 2024. PMID: 38793651 Free PMC article. Review. - Projection-TAGs enable multiplex projection tracing and multi-modal profiling of projection neurons.
Yang L, Liu F, Hahm H, Okuda T, Li X, Zhang Y, Kalyanaraman V, Heitmeier MR, Samineni VK. Yang L, et al. bioRxiv [Preprint]. 2024 Apr 28:2024.04.24.590975. doi: 10.1101/2024.04.24.590975. bioRxiv. 2024. PMID: 38712231 Free PMC article. Preprint. - Morphological Tracing and Functional Identification of Monosynaptic Connections in the Brain: A Comprehensive Guide.
Li Y, Fang Y, Li K, Yang H, Duan S, Sun L. Li Y, et al. Neurosci Bull. 2024 Sep;40(9):1364-1378. doi: 10.1007/s12264-024-01196-0. Epub 2024 May 3. Neurosci Bull. 2024. PMID: 38700806 Free PMC article. Review. - Identification of a novel perifornical-hypothalamic-area-projecting serotonergic system that inhibits innate panic and conditioned fear responses.
Bernabe CS, Caliman IF, de Abreu ARR, Molosh AI, Truitt WA, Shekhar A, Johnson PL. Bernabe CS, et al. Transl Psychiatry. 2024 Jan 25;14(1):60. doi: 10.1038/s41398-024-02769-3. Transl Psychiatry. 2024. PMID: 38272876 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources