Securin is not required for cellular viability, but is required for normal growth of mouse embryonic fibroblasts - PubMed (original) (raw)
Securin is not required for cellular viability, but is required for normal growth of mouse embryonic fibroblasts
J Mei et al. Curr Biol. 2001.
Free article
Abstract
Sister chromatid separation depends on the release of cohesion by the activity of Esp1, a member of the caspase family [1, 2]. In budding yeast, Esp1p is kept inactive by its association with Pds1p, until the onset of anaphase, when Pds1p is ubiquitinated by the APC/Cdc20 complex [3--5] and subsequently degraded by the 26S proteasome. Pds1 is not an essential gene in budding yeast, but is required for cell cycle arrest prior to anaphase in response to the disruption of spindle structures [6, 7]. Thus, Pds1 mutant yeast cells display precocious sister chromatid separation in the presence of nocodazole [6]. Mammalian orthologs of yeast Esp1 and Pds1, separin and securin, have been identified [8], and, as anticipated, a nondegradable mutant form of securin inhibits sister separation when added to mitotic Xenopus egg extracts [8]. Securin was also independently identified as PTTG (pituitary tumor transforming gene), a gene overexpressed in pituitary tumors [9]. The relationship between its overexpression in tumors and its control of sister chromatid cohesion remains ill defined. To explore securin function in mammals, we took a targeted gene disruption approach in mice. Here, we report that securin is neither essential for cell viability nor required for spindle checkpoint function, and mice lacking securin are viable and apparently normal, but mouse embryonic fibroblasts lacking securin grow abnormally in culture.
Similar articles
- Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s).
Yamamoto A, Guacci V, Koshland D. Yamamoto A, et al. J Cell Biol. 1996 Apr;133(1):99-110. doi: 10.1083/jcb.133.1.99. J Cell Biol. 1996. PMID: 8601617 Free PMC article. - Destruction of the securin Pds1p occurs at the onset of anaphase during both meiotic divisions in yeast.
Salah SM, Nasmyth K. Salah SM, et al. Chromosoma. 2000;109(1-2):27-34. doi: 10.1007/s004120050409. Chromosoma. 2000. PMID: 10855492 - A novel role of the budding yeast separin Esp1 in anaphase spindle elongation: evidence that proper spindle association of Esp1 is regulated by Pds1.
Jensen S, Segal M, Clarke DJ, Reed SI. Jensen S, et al. J Cell Biol. 2001 Jan 8;152(1):27-40. doi: 10.1083/jcb.152.1.27. J Cell Biol. 2001. PMID: 11149918 Free PMC article. - Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis.
Zou H, McGarry TJ, Bernal T, Kirschner MW. Zou H, et al. Science. 1999 Jul 16;285(5426):418-22. doi: 10.1126/science.285.5426.418. Science. 1999. PMID: 10411507 - Cyclin A and Nek2A: APC/C-Cdc20 substrates invisible to the mitotic spindle checkpoint.
van Zon W, Wolthuis RM. van Zon W, et al. Biochem Soc Trans. 2010 Feb;38(Pt 1):72-7. doi: 10.1042/BST0380072. Biochem Soc Trans. 2010. PMID: 20074038 Review.
Cited by
- Hexavalent Chromium Targets Securin to Drive Numerical Chromosome Instability in Human Lung Cells.
Toyoda JH, Martino J, Speer RM, Meaza I, Lu H, Williams AR, Bolt AM, Kouokam JC, Aboueissa AE, Wise JP Sr. Toyoda JH, et al. Int J Mol Sci. 2023 Dec 23;25(1):256. doi: 10.3390/ijms25010256. Int J Mol Sci. 2023. PMID: 38203427 Free PMC article. - Separase and Roads to Disengage Sister Chromatids during Anaphase.
Konecna M, Abbasi Sani S, Anger M. Konecna M, et al. Int J Mol Sci. 2023 Feb 27;24(5):4604. doi: 10.3390/ijms24054604. Int J Mol Sci. 2023. PMID: 36902034 Free PMC article. Review. - Separase Control and Cohesin Cleavage in Oocytes: Should I Stay or Should I Go?
Wassmann K. Wassmann K. Cells. 2022 Oct 27;11(21):3399. doi: 10.3390/cells11213399. Cells. 2022. PMID: 36359795 Free PMC article. Review. - DNA damage checkpoint execution and the rules of its disengagement.
Yam CQX, Lim HH, Surana U. Yam CQX, et al. Front Cell Dev Biol. 2022 Oct 6;10:1020643. doi: 10.3389/fcell.2022.1020643. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36274841 Free PMC article. Review. - Accumulation of Securin on Spindle During Female Meiosis I.
Pauerova T, Radonova L, Horakova A, Knott JG, Anger M. Pauerova T, et al. Front Cell Dev Biol. 2021 Jul 29;9:701179. doi: 10.3389/fcell.2021.701179. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34395431 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases