Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p - PubMed (original) (raw)

. 2001 Jul;39(5-6):284-90.

doi: 10.1007/s002940100229.

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• PMID: 11525400
• DOI: 10.1007/s002940100229

Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p

K Kerkmann et al. Curr Genet. 2001 Jul.

Abstract

Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a strain deleted for CDC73 on a genome-wide scale revealed that Cdc73p is involved in the positive and negative regulation of transcription. Our results show that a subset of genes is regulated by both Cdc73p and Tup1p. Furthermore, our results indicate that Cdc73p plays a role in the repression of telomere-proximal genes, which are not repressed by Tup1p.

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