Nuclear localization of EGF receptor and its potential new role as a transcription factor - PubMed (original) (raw)
Nuclear localization of EGF receptor and its potential new role as a transcription factor
S Y Lin et al. Nat Cell Biol. 2001 Sep.
Abstract
Epidermal growth factor receptor (EGFR) has been detected in the nucleus in many tissues and cell lines. However, the potential functions of nuclear EGFR have largely been overlooked. Here we demonstrate that nuclear EGFR is strongly correlated with highly proliferating activities of tissues. When EGFR was fused to the GAL4 DNA-binding domain, we found that the carboxy terminus of EGFR contained a strong transactivation domain. Moreover, the receptor complex bound and activated AT-rich consensus-sequence-dependent transcription, including the consensus site in cyclin D1 promoter. By using chromatin immunoprecipitation assays, we further demonstrated that nuclear EGFR associated with promoter region of cyclin D1 in vivo. EGFR might therefore function as a transcription factor to activate genes required for highly proliferating activities.
Comment in
- EGF receptors as transcription factors: ridiculous or sublime?
Waugh MG, Hsuan JJ. Waugh MG, et al. Nat Cell Biol. 2001 Sep;3(9):E209-11. doi: 10.1038/ncb0901-e209. Nat Cell Biol. 2001. PMID: 11533675 - Localizing the EGF receptor.
Oksvold M, Huitfeldt H, Stang E, Madshus I. Oksvold M, et al. Nat Cell Biol. 2002 Feb;4(2):E22; author reply E22-3. doi: 10.1038/ncb0202-e22a. Nat Cell Biol. 2002. PMID: 11835048 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous