Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels - PubMed (original) (raw)
Affiliations
- PMID: 11556581
Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels
P Eamsobhana et al. Southeast Asian J Trop Med Public Health. 2001 Jun.
Abstract
A 31-kDa glycoprotein antigen was purified by electrophoresing the crude extract of Parastrongylus cantonensis adult worms in a 12% SDS-polyacrylamide gel, identifying the 31-kDa component with prestained molecular weight standards, cutting the desired gel strip, and then isolating it by electroelution. Antigen fraction of 31 kDa was re-electrophoresed, transferred to a nitrocellulose membrane and found to be reactive with only the sera from patients with parastrongyliasis. No reactive band was observed with the sera from other related parasitic infections, eg, gnathostomiasis, toxocariasis, filariasis, paragonimiasis, cysticercosis and malaria, and the normal healthy control sera. This antigen fraction isolated showed 100% sensitivity and 100% specificity in the enzyme-linked immunosorbent assay (ELISA) for the detection of 31-kDa specific antibody in the sera from patients with parastrongyliasis. The P. cantonensis antigen of 31 kDa has been obtained by this means with a high degree of purity and applied successfully in conventional ELISA for the specific immunodiagnosis of human parastrongyliasis.
Similar articles
- Detection of circulating antigens of Parastrongylus cantonensis in human sera by dot-blot ELISA and sandwich ELISA using monoclonal antibody.
Eamsobhana P, Yong HS, Mak JW, Wattanakulpanich D. Eamsobhana P, et al. Southeast Asian J Trop Med Public Health. 1997 Sep;28(3):624-8. Southeast Asian J Trop Med Public Health. 1997. PMID: 9561620 - Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody.
Eamsobhana P, Mak JW, Yong HS. Eamsobhana P, et al. Southeast Asian J Trop Med Public Health. 1995 Dec;26(4):712-5. Southeast Asian J Trop Med Public Health. 1995. PMID: 9139382 - Detection of circulating antigens of Parastrongylus cantonensis in human sera by sandwich ELISA with specific monoclonal antibody.
Eamsobhana P, Mak JW, Yong HS. Eamsobhana P, et al. Southeast Asian J Trop Med Public Health. 1997;28 Suppl 1:139-42. Southeast Asian J Trop Med Public Health. 1997. PMID: 9656365 - Immunological diagnosis of human angiostrongyliasis due to Angiostrongylus cantonensis (Nematoda: Angiostrongylidae).
Eamsobhana P, Yong HS. Eamsobhana P, et al. Int J Infect Dis. 2009 Jul;13(4):425-31. doi: 10.1016/j.ijid.2008.09.021. Epub 2008 Dec 30. Int J Infect Dis. 2009. PMID: 19117782 Review. - Third report of ocular parastrongyliasis (angiostrongyliasis) from Sri Lanka.
Dissanaike AS, Ihalamulla RL, Naotunne TS, Senarathna T, Withana DS. Dissanaike AS, et al. Parassitologia. 2001 Sep;43(3):95-7. Parassitologia. 2001. PMID: 11921545 Review.
Cited by
- Rapid Single-Step Immunochromatographic Assay for Angiostrongylus cantonensis Specific Antigen Detection.
Eamsobhana P, Tungtrongchitr A, Wanachiwanawin D, Boonyong S, Yong HS. Eamsobhana P, et al. Pathogens. 2023 May 25;12(6):762. doi: 10.3390/pathogens12060762. Pathogens. 2023. PMID: 37375452 Free PMC article. - Immunodiagnostic Detection of Angiostrongylus cantonensis Exposure on Hawaii Island Using Isogeographic 31-kDa Antigen.
Jarvi SI, Nakayama K, Eamsobhana P, Kaluna L, Shepherd L, Tagami Y. Jarvi SI, et al. Am J Trop Med Hyg. 2023 Jun 12;109(2):335-342. doi: 10.4269/ajtmh.22-0643. Print 2023 Aug 2. Am J Trop Med Hyg. 2023. PMID: 37308099 Free PMC article. - Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis.
Eamsobhana P, Tungtrongchitr A, Yong HS, Prasartvit A, Wanachiwanawin D, Gan XX. Eamsobhana P, et al. Parasitology. 2021 Feb;148(2):234-239. doi: 10.1017/S0031182020001894. Epub 2020 Oct 2. Parasitology. 2021. PMID: 33004092 Free PMC article. - Estimating Human Exposure to Rat Lungworm (Angiostrongylus cantonensis) on Hawai'i Island: A Pilot Study.
Jarvi SI, Eamsobhana P, Quarta S, Howe K, Jacquier S, Hanlon A, Snook K, McHugh R, Tman Z, Miyamura J, Kramer K, Meyer M. Jarvi SI, et al. Am J Trop Med Hyg. 2020 Jan;102(1):69-77. doi: 10.4269/ajtmh.18-0242. Am J Trop Med Hyg. 2020. PMID: 31769399 Free PMC article.