Collagenase-3 expression in breast myofibroblasts as a molecular marker of transition of ductal carcinoma in situ lesions to invasive ductal carcinomas - PubMed (original) (raw)
. 2001 Oct 1;61(19):7091-100.
Affiliations
- PMID: 11585740
Collagenase-3 expression in breast myofibroblasts as a molecular marker of transition of ductal carcinoma in situ lesions to invasive ductal carcinomas
B S Nielsen et al. Cancer Res. 2001.
Abstract
Collagenase-3 (matrix metalloproteinase 13; MMP-13), a protease originally identified in breast carcinoma, is characterized by a potent degrading activity against a wide spectrum of extracellular matrix proteins. The aims of this study were to localize and identify the MMP-13-expressing cells in invasive human breast carcinoma and to evaluate the role of MMP-13 in transition to invasive lesions by studying ductal carcinoma in situ (DCIS). We found expression of MMP-13 in stromal fibroblast-like cells in all 21 invasive ductal carcinomas studied and in 4 of 9 invasive lobular carcinomas. In most carcinomas, expression of MMP-13 was limited to small stromal foci in the tumor area. Combined in situ hybridization and immunohistochemistry showed coexpression of alpha-smooth muscle actin immunoreactivity and MMP-13 mRNA in myofibroblasts. In contrast, cytokeratin-positive cancer cells, alpha-smooth muscle actin-positive vascular smooth muscle cells, CD68-positive macrophages, and CD31-positive endothelial cells were all MMP-13 mRNA negative. In situ hybridization for MMP-13 in 17 DCIS lesions revealed expression in 10 cases. Immunohistochemical analysis of all DCIS cases identified microinvasion in 8 of the 17 lesions. Seven of the eight lesions with microinvasion were MMP-13 positive. Further analysis showed that MMP-13 expression was often associated with the microinvasive events. This particular expression pattern was unique for MMP-13 among other MMPs analyzed, including MMP-2, -11, and -14. We conclude that MMP-13 is primarily expressed by myofibroblasts in human breast carcinoma and that expression in DCIS lesions often is associated with microinvasive events. On the basis of these data, we propose that MMP-13 may play an essential role during transition of DCIS lesions to invasive ductal carcinomas.
Similar articles
- Messenger RNA for urokinase plasminogen activator is expressed in myofibroblasts adjacent to cancer cells in human breast cancer.
Nielsen BS, Sehested M, Timshel S, Pyke C, Danø K. Nielsen BS, et al. Lab Invest. 1996 Jan;74(1):168-77. Lab Invest. 1996. PMID: 8569179 - Expression of matrix metalloprotease-9 in vascular pericytes in human breast cancer.
Nielsen BS, Sehested M, Kjeldsen L, Borregaard N, Rygaard J, Danø K. Nielsen BS, et al. Lab Invest. 1997 Oct;77(4):345-55. Lab Invest. 1997. PMID: 9354769 - Ductal carcinoma in situ of the breast and heparanase-1 expression: a molecular explanation for more aggressive subtypes.
Maxhimer JB, Pesce CE, Stewart RA, Gattuso P, Prinz RA, Xu X. Maxhimer JB, et al. J Am Coll Surg. 2005 Mar;200(3):328-35. doi: 10.1016/j.jamcollsurg.2004.10.034. J Am Coll Surg. 2005. PMID: 15737842 - Epidemiological survey of preinvasive breast cancer.
Vandenbroucke A, Bourdon C. Vandenbroucke A, et al. Eur J Cancer Prev. 1993 Nov;2 Suppl 3:3-10. Eur J Cancer Prev. 1993. PMID: 8298449 Review.
Cited by
- Targets in the Tumour Matrisome to Promote Cancer Therapy Response.
Jalil SMA, Henry JC, Cameron AJM. Jalil SMA, et al. Cancers (Basel). 2024 May 11;16(10):1847. doi: 10.3390/cancers16101847. Cancers (Basel). 2024. PMID: 38791926 Free PMC article. Review. - TGFβ-mediated MMP13 secretion drives myoepithelial cell dependent breast cancer progression.
Gibson SV, Tomas Bort E, Rodríguez-Fernández L, Allen MD, Gomm JJ, Goulding I, Auf dem Keller U, Agnoletto A, Brisken C, Peck B, Cameron AJ, Marshall JF, Jones JL, Carter EP, Grose RP. Gibson SV, et al. NPJ Breast Cancer. 2023 Mar 2;9(1):9. doi: 10.1038/s41523-023-00513-6. NPJ Breast Cancer. 2023. PMID: 36864079 Free PMC article. - Mechanostimulation of breast myoepithelial cells induces functional changes associated with DCIS progression to invasion.
Hayward MK, Allen MD, Gomm JJ, Goulding I, Thompson CL, Knight MM, Marshall JF, Jones JL. Hayward MK, et al. NPJ Breast Cancer. 2022 Sep 20;8(1):109. doi: 10.1038/s41523-022-00464-4. NPJ Breast Cancer. 2022. PMID: 36127361 Free PMC article. - STAT3 induces breast cancer growth via ANGPTL4, MMP13 and STC1 secretion by cancer associated fibroblasts.
Avalle L, Raggi L, Monteleone E, Savino A, Viavattene D, Statello L, Camperi A, Stabile SA, Salemme V, De Marzo N, Marino F, Guglielmi C, Lobascio A, Zanini C, Forni M, Incarnato D, Defilippi P, Oliviero S, Poli V. Avalle L, et al. Oncogene. 2022 Mar;41(10):1456-1467. doi: 10.1038/s41388-021-02172-y. Epub 2022 Jan 18. Oncogene. 2022. PMID: 35042959 - Tumor-Derived Matrix Metalloproteinase-13 (MMP-13) Expression in Benign and Malignant Breast Lesions.
Ameli F, Ghafourina Nassab F, Masir N, Kahtib F. Ameli F, et al. Asian Pac J Cancer Prev. 2021 Aug 1;22(8):2603-2609. doi: 10.31557/APJCP.2021.22.8.2603. Asian Pac J Cancer Prev. 2021. PMID: 34452576 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Medical
Miscellaneous