Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium - PubMed (original) (raw)

Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium

G Werner et al. Antimicrob Agents Chemother. 2001 Nov.

Abstract

Seventy-two Enterococcus faecium isolates of different origins highly resistant to nourseothricin and streptomycin were studied. Sequencing of a genomic fragment from two isolates identified a gene cluster, aadE-sat4-aphA-3, which has been isolated recently in staphylococci and Campylobacter coli. Patterns of digested PCR products of aadE-sat4-aphA-3 were identical for all isolates.

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Figures

FIG. 1

Distribution of MICs of nourseothricin among 95 E. faecium isolates.

FIG. 2

Genetic structure of the three open reading frames (ORFs) from the sequenced fragments of E. faecium UW786 and UW1965. Putative amino acid sequence of ORF1 to -3 is shown in accordance with results from databank screens (100% identical to parts of GenBank accession number AF299292). Restriction endonuclease sites for _Dde_I (CTNAG) are shown. Small black arrows indicate the binding sites for the two internal primers, aadE-1 and aphA-2. The start codon of ORF2 (sat4) overlaps with the stop codon of ORF1 (aadE). The complete nucleotide composition and putative protein sequences are given in GenBank under accession number AF330699.

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Aminoglycoside-streptothricin resistance gene cluster aadE-sat4-aphA-3 disseminated among multiresistant isolates of Enterococcus faecium - PubMed (original) (raw)