Identification of a novel tetramerization domain in large conductance K(ca) channels - PubMed (original) (raw)

Identification of a novel tetramerization domain in large conductance K(ca) channels

J C Quirk et al. Neuron. 2001.

Free article

Abstract

More than 50 genes are known to encode K(+) channel monomers and can coassemble to form hetero-tetrameric K(+) channels. However, only a subset of possible monomer combinations come together to form functional ion channels. The assembly and tetramerization of appropriate channel monomers is mediated by association domains (ADs). To identify such domains in human large-conductance Ca(2+)-activated K(+) channels (hSlo1), we screened hSlo1 domains for self-association using yeast two-hybrid assays. Putative ADs were subjected to functional assays in Xenopus oocytes and further characterized by coprecipitation, native gel electrophoresis, and sucrose density gradient centrifugation assays. This led to the identification of a single intracellular association domain localized near the channel pore and required for channel function. We conclude that this novel tetramerization domain, referred to as BK-T1, promotes the assembly of hSlo1 monomers into functional K(Ca) channels.

PubMed Disclaimer

Cited by

Dominant-negative regulation of cell surface expression by a pentapeptide motif at the extreme COOH terminus of an Slo1 calcium-activated potassium channel splice variant.
Chiu YH, Alvarez-Baron C, Kim EY, Dryer SE. Chiu YH, et al. Mol Pharmacol. 2010 Apr;77(4):497-507. doi: 10.1124/mol.109.061929. Epub 2010 Jan 5. Mol Pharmacol. 2010. PMID: 20051533 Free PMC article.
Palmitoylation and membrane association of the stress axis regulated insert (STREX) controls BK channel regulation by protein kinase C.
Zhou X, Wulfsen I, Korth M, McClafferty H, Lukowski R, Shipston MJ, Ruth P, Dobrev D, Wieland T. Zhou X, et al. J Biol Chem. 2012 Sep 14;287(38):32161-71. doi: 10.1074/jbc.M112.386359. Epub 2012 Jul 29. J Biol Chem. 2012. PMID: 22843729 Free PMC article.
Translocation of an endoproteolytically cleaved maxi-K channel isoform: mechanisms to induce human myometrial cell repolarization.
Korovkina VP, Brainard AM, England SK. Korovkina VP, et al. J Physiol. 2006 Jun 1;573(Pt 2):329-41. doi: 10.1113/jphysiol.2006.106922. Epub 2006 Mar 9. J Physiol. 2006. PMID: 16527852 Free PMC article.
Voltage-dependent dynamics of the BK channel cytosolic gating ring are coupled to the membrane-embedded voltage sensor.
Miranda P, Holmgren M, Giraldez T. Miranda P, et al. Elife. 2018 Dec 11;7:e40664. doi: 10.7554/eLife.40664. Elife. 2018. PMID: 30526860 Free PMC article.
Defining the BK channel domains required for beta1-subunit modulation.
Morrow JP, Zakharov SI, Liu G, Yang L, Sok AJ, Marx SO. Morrow JP, et al. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5096-101. doi: 10.1073/pnas.0600907103. Epub 2006 Mar 20. Proc Natl Acad Sci U S A. 2006. PMID: 16549765 Free PMC article.

Identification of a novel tetramerization domain in large conductance K(ca) channels - PubMed (original) (raw)