Rab5 association with the angiotensin II type 1A receptor promotes Rab5 GTP binding and vesicular fusion - PubMed (original) (raw)
. 2002 Jan 4;277(1):679-85.
doi: 10.1074/jbc.M109022200. Epub 2001 Oct 26.
Affiliations
- PMID: 11682489
- DOI: 10.1074/jbc.M109022200
Free article
Rab5 association with the angiotensin II type 1A receptor promotes Rab5 GTP binding and vesicular fusion
Jennifer L Seachrist et al. J Biol Chem. 2002.
Free article
Abstract
Previous studies have demonstrated that the internalization of the angiotensin II type 1A receptor (AT(1A)R) may be mediated by both beta-arrestin-sensitive and -insensitive mechanisms. Therefore, we have used the AT(1A)R carboxyl-terminal tail to screen a rat brain yeast two-hybrid expression library for novel AT(1A)R-interacting proteins that might contribute to the regulation of AT(1A)R internalization. We have identified Rab5a as an AT(1A)R-binding protein that selectively associates with the AT(1A)R and not with the beta2-adrenergic receptor. A Rab5a-S34N mutant defective in GTP binding does not prevent the internalization of the AT(1A)R but does prevent the trafficking of the AT(1A)R into larger hollow cored vesicular structures. Agonist activation of the AT(1A)R promotes both the formation of Rab5a.AT(1A)R protein complexes and Rab5a GTP binding. Rab5a interactions with the AT(1A)R are mediated in part by the last 10 amino acid residues of the AT(1A)R carboxyl-terminal tail, and although a mutant receptor lacking these residues internalizes normally, it does not redistribute into larger hollow vesicles. Our data suggest that AT(1A)R activation modulates Rab5a activity leading to the homotypic fusion of endocytic vesicles. These observations suggest that vesicular cargo proteins, such as the AT(1A)R, may control their targeting between intracellular compartments by directly regulating the activity of components of the intracellular trafficking machinery such as Rab5a.
Similar articles
- Regulation of angiotensin II type 1A receptor intracellular retention, degradation, and recycling by Rab5, Rab7, and Rab11 GTPases.
Dale LB, Seachrist JL, Babwah AV, Ferguson SS. Dale LB, et al. J Biol Chem. 2004 Mar 26;279(13):13110-8. doi: 10.1074/jbc.M313333200. Epub 2004 Jan 7. J Biol Chem. 2004. PMID: 14711821 - Epidermal growth factor and membrane trafficking. EGF receptor activation of endocytosis requires Rab5a.
Barbieri MA, Roberts RL, Gumusboga A, Highfield H, Alvarez-Dominguez C, Wells A, Stahl PD. Barbieri MA, et al. J Cell Biol. 2000 Oct 30;151(3):539-50. doi: 10.1083/jcb.151.3.539. J Cell Biol. 2000. PMID: 11062256 Free PMC article. - Receptor/beta-arrestin complex formation and the differential trafficking and resensitization of beta2-adrenergic and angiotensin II type 1A receptors.
Anborgh PH, Seachrist JL, Dale LB, Ferguson SS. Anborgh PH, et al. Mol Endocrinol. 2000 Dec;14(12):2040-53. doi: 10.1210/mend.14.12.0565. Mol Endocrinol. 2000. PMID: 11117533 - Molecular mechanisms of angiotensin II receptor internalization.
Hunyady L. Hunyady L. J Am Soc Nephrol. 1999 Jan;10 Suppl 11:S47-56. J Am Soc Nephrol. 1999. PMID: 9892140 Review. - Direct interactions between rab GTPases and cargo.
Smythe E. Smythe E. Mol Cell. 2002 Feb;9(2):205-6. doi: 10.1016/s1097-2765(02)00462-8. Mol Cell. 2002. PMID: 11864591 Review.
Cited by
- The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence.
Follit JA, Li L, Vucica Y, Pazour GJ. Follit JA, et al. J Cell Biol. 2010 Jan 11;188(1):21-8. doi: 10.1083/jcb.200910096. Epub 2010 Jan 4. J Cell Biol. 2010. PMID: 20048263 Free PMC article. - New insights in the regulation of Rab GTPases by G protein-coupled receptors.
Lachance V, Angers S, Parent JL. Lachance V, et al. Small GTPases. 2014;5:e29039. doi: 10.4161/sgtp.29039. Epub 2014 May 9. Small GTPases. 2014. PMID: 24950538 Free PMC article. - International Union of Basic and Clinical Pharmacology. XCIX. Angiotensin Receptors: Interpreters of Pathophysiological Angiotensinergic Stimuli [corrected].
Karnik SS, Unal H, Kemp JR, Tirupula KC, Eguchi S, Vanderheyden PM, Thomas WG. Karnik SS, et al. Pharmacol Rev. 2015 Oct;67(4):754-819. doi: 10.1124/pr.114.010454. Pharmacol Rev. 2015. PMID: 26315714 Free PMC article. Review. - Investigation of the fate of type I angiotensin receptor after biased activation.
Szakadáti G, Tóth AD, Oláh I, Erdélyi LS, Balla T, Várnai P, Hunyady L, Balla A. Szakadáti G, et al. Mol Pharmacol. 2015 Jun;87(6):972-81. doi: 10.1124/mol.114.097030. Epub 2015 Mar 24. Mol Pharmacol. 2015. PMID: 25804845 Free PMC article. - Endothelin-converting enzyme-1 degrades internalized somatostatin-14.
Roosterman D, Kempkes C, Cottrell GS, Padilla BE, Bunnett NW, Turck CW, Steinhoff M. Roosterman D, et al. Endocrinology. 2008 May;149(5):2200-7. doi: 10.1210/en.2007-1628. Epub 2008 Feb 14. Endocrinology. 2008. PMID: 18276747 Free PMC article.