Physical mapping across an avirulence locus of Phytophthora infestans using a highly representative, large-insert bacterial artificial chromosome library - PubMed (original) (raw)
. 2001 Oct;266(2):289-95.
doi: 10.1007/s004380100555.
Affiliations
- PMID: 11683271
- DOI: 10.1007/s004380100555
Physical mapping across an avirulence locus of Phytophthora infestans using a highly representative, large-insert bacterial artificial chromosome library
S C Whisson et al. Mol Genet Genomics. 2001 Oct.
Abstract
The oomycete plant pathogen Phytophthora infestans is the causal agent of late blight, one of the most devastating diseases of potato worldwide. As part of efforts to clone avirulence (Avr) genes and pathogenicity factors from P. infestans, we have constructed a bacterial artificial chromosome (BAC) library from an isolate containing six Avr genes. The BAC library comprises clones with an average insert size of 98 kb and represents an estimated 10 genome equivalents. A three-dimensional pooling strategy was developed to screen the BAC library for amplified fragment length polymorphism (AFLP) markers, as this type of marker has been extensively used in construction of a P. infestans genetic map. Multiple positive clones were identified for each AFLP marker tested. The pools were used to construct a contig of 11 BAC clones in a region of the P. infestans genome containing a cluster of three avirulence genes. The BAC contig is predicted to encompass the Avr11 locus but mapping of the BAC ends will be required to determine if the Avr3 and Avr10 loci are also present in the BAC contig. These results are an important step towards the positional cloning of avirulence genes from P. infestans, and the BAC library represents a valuable resource for largescale studies of oomycete genome organisation and gene content.
Similar articles
- Amplification generates modular diversity at an avirulence locus in the pathogen Phytophthora.
Jiang RH, Weide R, van de Vondervoort PJ, Govers F. Jiang RH, et al. Genome Res. 2006 Jul;16(7):827-40. doi: 10.1101/gr.5193806. Genome Res. 2006. PMID: 16818726 Free PMC article. - A genetic interval and physical contig spanning the Peronospora parasitica (At) avirulence gene locus ATR1Nd.
Rehmany AP, Grenville LJ, Gunn ND, Allen RL, Paniwnyk Z, Byrne J, Whisson SC, Birch PR, Beynon JL. Rehmany AP, et al. Fungal Genet Biol. 2003 Feb;38(1):33-42. doi: 10.1016/s1087-1845(02)00515-7. Fungal Genet Biol. 2003. PMID: 12553934 - Mapping of avirulence genes in Phytophthora infestans with amplified fragment length polymorphism markers selected by bulked segregant analysis.
van der Lee T, Robold A, Testa A, van 't Klooster JW, Govers F. van der Lee T, et al. Genetics. 2001 Mar;157(3):949-56. doi: 10.1093/genetics/157.3.949. Genetics. 2001. PMID: 11238385 Free PMC article. - Effector-triggered immunity by the plant pathogen Phytophthora.
Qutob D, Tedman-Jones J, Gijzen M. Qutob D, et al. Trends Microbiol. 2006 Nov;14(11):470-3. doi: 10.1016/j.tim.2006.09.004. Epub 2006 Sep 25. Trends Microbiol. 2006. PMID: 16996740 Review. - Pathogen virulence of Phytophthora infestans: from gene to functional genomics.
Sanju S, Thakur A, Siddappa S, Sreevathsa R, Srivastava N, Shukla P, Singh BP. Sanju S, et al. Physiol Mol Biol Plants. 2013 Apr;19(2):165-77. doi: 10.1007/s12298-012-0157-z. Physiol Mol Biol Plants. 2013. PMID: 24431484 Free PMC article. Retracted. Review.
Cited by
- Amplification generates modular diversity at an avirulence locus in the pathogen Phytophthora.
Jiang RH, Weide R, van de Vondervoort PJ, Govers F. Jiang RH, et al. Genome Res. 2006 Jul;16(7):827-40. doi: 10.1101/gr.5193806. Genome Res. 2006. PMID: 16818726 Free PMC article. - Sequence diversity in the large subunit of RNA polymerase I contributes to Mefenoxam insensitivity in Phytophthora infestans.
Randall E, Young V, Sierotzki H, Scalliet G, Birch PR, Cooke DE, Csukai M, Whisson SC. Randall E, et al. Mol Plant Pathol. 2014 Sep;15(7):664-76. doi: 10.1111/mpp.12124. Epub 2014 Apr 14. Mol Plant Pathol. 2014. PMID: 24521429 Free PMC article. - An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm.
Armstrong MR, Whisson SC, Pritchard L, Bos JI, Venter E, Avrova AO, Rehmany AP, Böhme U, Brooks K, Cherevach I, Hamlin N, White B, Fraser A, Lord A, Quail MA, Churcher C, Hall N, Berriman M, Huang S, Kamoun S, Beynon JL, Birch PR. Armstrong MR, et al. Proc Natl Acad Sci U S A. 2005 May 24;102(21):7766-71. doi: 10.1073/pnas.0500113102. Epub 2005 May 13. Proc Natl Acad Sci U S A. 2005. PMID: 15894622 Free PMC article. - Elicitin genes in Phytophthora infestans are clustered and interspersed with various transposon-like elements.
Jiang RH, Dawe AL, Weide R, van Staveren M, Peters S, Nuss DL, Govers F. Jiang RH, et al. Mol Genet Genomics. 2005 Mar;273(1):20-32. doi: 10.1007/s00438-005-1114-0. Epub 2005 Feb 9. Mol Genet Genomics. 2005. PMID: 15702346 - Marker-based cloning of the region containing the UhAvr1 avirulence gene from the basidiomycete barley pathogen Ustilago hordei.
Linning R, Lin D, Lee N, Abdennadher M, Gaudet D, Thomas P, Mills D, Kronstad JW, Bakkeren G. Linning R, et al. Genetics. 2004 Jan;166(1):99-111. doi: 10.1534/genetics.166.1.99. Genetics. 2004. PMID: 15020410 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials