Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division - PubMed (original) (raw)

Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division

B Feierbach et al. Curr Biol. 2001.

Free article

Abstract

Background: Both symmetric and asymmetric cell divisions are required for the generation of appropriate cell lineages during development. Wild-type Schizosaccharomyces pombe cells divide in a symmetric fashion to produce two similar rod-shaped daughter cells. Formins are proteins with conserved roles in cell polarity, cytokinesis, and the regulation of actin and microtubule cytoskeletons.

Results: Here, we identify and characterize a new S. pombe formin, for3p. for3 Delta mutant cells divide in an asymmetric manner; a mother cell divides medially to produce one daughter cell that develops into a monopolar cell and one daughter that develops into a bipolar cell. Both daughter cells recapitulate similar asymmetric lineages themselves. Inheritance of the bipolar pattern correlates with inheritance of the recent birth scar, not with asymmetry in the spindle pole bodies. for3 Delta mutants lack interphase actin cables and have delocalized actin patch and myo52p (type V myosin) distributions. for3 Delta cells have normal microtubule dynamics and cortical interactions but have defects in microtubule organization and increased numbers of microtubule bundles. for3p-GFP is localized at both cell tips in an actin-dependent manner and at the cell division site.

Conclusions: for3p is a cell polarity factor required for interphase actin cable formation and microtubule organization. The for3 Delta phenotype suggests that cells are able to grow in a polarized manner even in the absence of functional actin cables and polarized distribution of actin patches. for3p and possibly actin cables are part of a regulatory network that ensures that cell divisions are symmetric.

PubMed Disclaimer

Comment in

Cell polarity: following formin function.
Sawin KE. Sawin KE. Curr Biol. 2002 Jan 8;12(1):R6-8. doi: 10.1016/s0960-9822(01)00637-6. Curr Biol. 2002. PMID: 11790314

Cited by

Actin turnover protects the cytokinetic contractile ring from structural instability.
McDargh Z, Zhu T, Zhu H, O'Shaughnessy B. McDargh Z, et al. J Cell Sci. 2023 Mar 1;136(5):jcs259969. doi: 10.1242/jcs.259969. Epub 2022 Oct 6. J Cell Sci. 2023. PMID: 36052670 Free PMC article.
Regulation of fission yeast morphogenesis by PP2A activator pta2.
Bernal M, Sanchez-Romero MA, Salas-Pino S, Daga RR. Bernal M, et al. PLoS One. 2012;7(3):e32823. doi: 10.1371/journal.pone.0032823. Epub 2012 Mar 5. PLoS One. 2012. PMID: 22403715 Free PMC article.
Shaping fission yeast with microtubules.
Chang F, Martin SG. Chang F, et al. Cold Spring Harb Perspect Biol. 2009 Jul;1(1):a001347. doi: 10.1101/cshperspect.a001347. Cold Spring Harb Perspect Biol. 2009. PMID: 20066076 Free PMC article. Review.
Mechanism controlling perpendicular alignment of the spindle to the axis of cell division in fission yeast.
Gachet Y, Tournier S, Millar JB, Hyams JS. Gachet Y, et al. EMBO J. 2004 Mar 24;23(6):1289-300. doi: 10.1038/sj.emboj.7600156. Epub 2004 Mar 11. EMBO J. 2004. PMID: 15014440 Free PMC article.
Cortical regulation of cell size by a sizer cdr2p.
Pan KZ, Saunders TE, Flor-Parra I, Howard M, Chang F. Pan KZ, et al. Elife. 2014 Mar 18;3:e02040. doi: 10.7554/eLife.02040. Elife. 2014. PMID: 24642412 Free PMC article.

Roles of the fission yeast formin for3p in cell polarity, actin cable formation and symmetric cell division - PubMed (original) (raw)