Mad2-Independent inhibition of APCCdc20 by the mitotic checkpoint protein BubR1 - PubMed (original) (raw)
Mad2-Independent inhibition of APCCdc20 by the mitotic checkpoint protein BubR1
Z Tang et al. Dev Cell. 2001 Aug.
Free article
Abstract
The mitotic checkpoint blocks the activation of the anaphase-promoting complex (APC) until all sister chromatids have achieved bipolar attachment to the spindle. A checkpoint complex containing BubR1 and Bub3 has been purified from mitotic human cells. Upon checkpoint activation, the BubR1-Bub3 complex interacts with Cdc20. In the absence of Mad2, BubR1 inhibits the activity of APC by blocking the binding of Cdc20 to APC. Surprisingly, the kinase activity of BubR1 is not required for the inhibition of APCCdc20. BubR1 also prevents the activation of APCCdc20 in Xenopus egg extracts, and restores the mitotic arrest in Cdc20-overexpressing cells treated with nocodazole. Because BubR1 also interacts with the mitotic motor CENP-E, the ability of BubR1 to inhibit APC may be regulated by kinetochore tension or occupancy.
Comment in
- Tracing the pathway of spindle assembly checkpoint signaling.
Gillett ES, Sorger PK. Gillett ES, et al. Dev Cell. 2001 Aug;1(2):162-4. doi: 10.1016/s1534-5807(01)00032-6. Dev Cell. 2001. PMID: 11702776 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous