Nuclear mislocalization of enzymatically active RanGAP causes segregation distortion in Drosophila - PubMed (original) (raw)
Nuclear mislocalization of enzymatically active RanGAP causes segregation distortion in Drosophila
A Kusano et al. Dev Cell. 2001 Sep.
Free article
Abstract
Segregation Distorter (SD) is a meiotic drive system in Drosophila that causes preferential transmission of the SD chromosome from SD/SD+ males owing to dysfunction of SD+ spermatids. The Sd locus, which is essential for distortion, encodes a truncated RanGAP (Ran GTPase activating protein), a key nuclear transport factor. Here, we show that Sd-RanGAP retains normal enzyme activity but is mislocalized to nuclei. Distortion is abolished when enzymatic activity or nuclear localization of Sd-RanGAP is perturbed. Overexpression of Ran or RanGEF (Ran GTPase exchange factor) in the male germline fully suppresses distortion. We conclude that mislocalization of Sd-RanGAP causes distortion by reducing nuclear RanGTP, thereby disrupting the Ran signaling pathway. Nuclear transport of a GFP reporter in salivary glands is impaired by SD, suggesting that a defect in nuclear transport may underlie sperm dysfunction.
Comment in
- Altered nuclear transport and the most selfish of genes.
Hawley RS. Hawley RS. Dev Cell. 2001 Sep;1(3):311-3. doi: 10.1016/s1534-5807(01)00050-8. Dev Cell. 2001. PMID: 11702939
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